Issue 9, 2004
From the journal:

Analyst

Spectrofluorimetric determination of surface-bound thiol groups and its application to the analysis of thiol-modified silicas

A. G. Howarda  and  N. H. Khdaryb  

a School of Chemistry, University of Southampton, Southampton, UK

b Ministry of Health, Makkah AL-Mukrammah, P.O. Box 8427, Saudi Arabia

Abstract

A sensitive spectofluorimetric method has been developed for the quantitative measurement of surface-bound thiol groups. The procedure is based on the quantitative esterification of the thiol group with Rhodamine B and its subsequent release from the solid by base hydrolysis for spectrofluorimetric determination. Application of the method to the analysis of thiol-modified nanoparticulate silicas yielded results that compared favourably against alternative approaches based on measurements of mercury capacity and iodometric titration of the thiol groups. Non-specific Rhodamine uptake, assessed using unmodified silica and C18-modified silica, did not significantly influence the analytical results. When applied to a typical 50 mg sample, the detection limit of the procedure was 1 nmole SH gāˆ’1 silica.

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Article information

DOI
https://doi.org/10.1039/B407566H
Article type
Paper
Submitted
19 May 2004
Accepted
12 Jul 2004
First published
04 Aug 2004

Analyst, 2004,129, 860-863

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Spectrofluorimetric determination of surface-bound thiol groups and its application to the analysis of thiol-modified silicas

A. G. Howard and N. H. Khdary, Analyst, 2004, 129, 860 DOI: 10.1039/B407566H

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