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The Analyst

The home of high impact research in analytical, bioanalytical and detection science.




Paper

Analyst, 2004, 129, 893 - 896, DOI: 10.1039/b408954p


Fast quantification of water in single living cells by near-infrared microscopy

Erik Bründermann, Andreas Bergner, Frank Petrat, Robert Schiwon, Götz Wollny, Ilona Kopf, Herbert de Groot and Martina Havenith


We have set up a near-infrared microscope using a tuneable diode laser in the range from 1530 to 1570 nm. This spectral range is close to the peak of the water overtone absorption. We used this new microscope to study liver cells, hepatocytes, showing that quantitative information of the intracellular water concentration in living cells can be extracted.