Issue 6, 2010

Microfluidic-based measurements of cytochrome P450enzyme activity of primary mammalian hepatocytes

Abstract

A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 μM and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of α-naphthoflavone was also examined on EROD activity resulting in an IC50 value of 12.98 μM.

Graphical abstract: Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

Article information

Article type
Paper
Submitted
24 Jan 2010
Accepted
19 Mar 2010
First published
17 Apr 2010

Analyst, 2010,135, 1282-1287

Microfluidic-based measurements of cytochrome P450 enzyme activity of primary mammalian hepatocytes

K. Anderson, J. M. Cooper, S. J. Haswell, D. Marshall, H. Yin and X. Zhang, Analyst, 2010, 135, 1282 DOI: 10.1039/C0AN00031K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements