Issue 12, 2010
From the journal:

Analyst

Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis†‡

Jemma G. Kelly,a   Takahiro Nakamura,b   Shigeru Kinoshita,b   Nigel J. Fullwoodc  and  Francis L. Martin*a  

* Corresponding authors

a Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Bailrigg, Lancaster, UK
E-mail: f.martin@lancaster.ac.uk

b Department of Ophthalmology, Kyoto Perfectural University of Medicine, Kawaramachi Hirohoji, Kamigyo-ku, Kyoto, Japan

c Biomedical and Life Sciences, School of Health and Medicine, Lancaster University, Lancaster, UK

Abstract

The cornea is one of the few human tissues where the in situ locations of stem cells (SCs), transient-amplifying (TA) cells and terminally-differentiated (TD) cells have been relatively well localised and characterised. Mid-infrared (IR) (4000–400 cm−1) is absorbed by biological molecules and facilitates the acquisition in the biochemical-cell fingerprint region (1800–900 cm−1) of spectra representative of structure and function. Human cornea derived from normal or squamous cell carcinoma (SCC) samples were acquired, cryosectioned (10 µm), floated onto BaF2 windows and interrogated using synchrotron-based radiation (SRS) Fourier-transform IR (FTIR) microspectroscopy. Spectra were analysed using principal component analysis (PCA) with or without linear discriminant analysis (LDA) to allow cluster analysis of the cell categories. A clear cell lineage emanating from SCs to TA cells to TD cells was noted in normal samples. Within the SCC samples, a small sub-population of the cell-derived spectra pointed to a SC-like phenotype with the vast majority pointing to a TAcell-like character; these cells would tend to be the most proliferative within a tissue. Our findings suggest that SRSFTIR microspectroscopy has the potential to identify and characterise cancer SCs.

Graphical abstract: Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis

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Article information

DOI
https://doi.org/10.1039/C0AN00507J
Article type
Paper
Submitted
14 Jul 2010
Accepted
24 Aug 2010
First published
01 Oct 2010

Analyst, 2010,135, 3120-3125

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Evidence for a stem-cell lineage in corneal squamous cell carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy and multivariate analysis

J. G. Kelly, T. Nakamura, S. Kinoshita, N. J. Fullwood and F. L. Martin, Analyst, 2010, 135, 3120 DOI: 10.1039/C0AN00507J

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