Cover image for Highlights in Chemical Biology

Highlights in Chemical Biology

Chemical biology news from across RSC Publishing.



Display Records 0 to 60 of 125 records
  

Contents list for Highlights in Chemical Biology: Research Articles, issue 9, 2009

hide images submit buttonRSS news feed image for latest articles

Review Articles

Dietary phenolics: chemistry, bioavailability and effects on health
Alan Crozier, Indu B. Jaganath and Michael N. Clifford,  Nat. Prod. Rep., 2009, 26, 1001
DOI: 10.1039/b802662a

graphical abstract image (ID: b802662a)

This review discusses dietary flavonoids and related phenolic compounds, their absorption, mammalian and microbial metabolism and how they might protect against chronic diseases. Attention is drawn to gaps in understanding.

Meroterpenoids produced by fungi
Regina Geris and Thomas J. Simpson,  Nat. Prod. Rep., 2009, 26, 1063
DOI: 10.1039/b820413f

graphical abstract image (ID: b820413f)

This review deals with the isolation and structure elucidation of meroterpenoids from higher and lower fungi, including discussion of biosynthetic studies and biological activities.

Chemistry and biology of non-tetramic -hydroxy--lactams and -alkylidene--lactams from natural sources
Bastien Nay, Nassima Riache and Laurent Evanno,  Nat. Prod. Rep., 2009, 26, 1044
DOI: 10.1039/b903905h

graphical abstract image (ID: b903905h)

Natural products containing non-tetramic -hydroxy--lactams and -alkylidene--lactams are usually but not exclusively derived from the mixed polyketide–non-ribosomal peptide biosynthetic pathway. This review intends to emphasize their structural originality, biological properties and the synthetic efforts developed to reach them.

Mirror image phage display—a method to generate D-peptide ligands for use in diagnostic or therapeutical applications
Susanne Aileen Funke and Dieter Willbold,  Mol. BioSyst., 2009, 5, 783
DOI: 10.1039/b904138a
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b904138a)

Mirror image phage display is an interesting approach to identify new D-enantiomeric peptide ligands. Here, examples of mirror image phage display derived D-peptides with therapeutical potential are introduced and discussed.

Locked nucleic acid nucleoside triphosphates and polymerases: on the way towards evolution of LNA aptamers
Rakesh N. Veedu and Jesper Wengel,  Mol. BioSyst., 2009, 5, 787
DOI: 10.1039/b905513b
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b905513b)

This article highlights the recent developments towards the enzymatic synthesis of LNA-containing oligonucleotides by incorporating in both DNA and RNA strands using LNA nucleoside 5-triphosphates and various polymerases.

Biomimetic approaches for studying membrane processes
Raz Jelinek and Liron Silbert,  Mol. BioSyst., 2009, 5, 811
DOI: 10.1039/b907223n
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b907223n)

Biomimetic membrane systems are currently employed for a variety of biochemical applications. This review summarizes recent developments in the design of chemical assemblies and techniques for studying membrane phenomena.

Physics puzzles on membrane domains posed by cell biology
Pierre-François Lenne and Alice Nicolas,  Soft Matter, 2009, 5, 2841
DOI: 10.1039/b822956b

graphical abstract image (ID: b822956b)

We review here recent studies on domains in adhering and non-adhering membranes and show that a broad range of biological studies challenge the concepts of membrane physics at thermodynamic equilibrium.

The complex biology of autocrine motility factor/phosphoglucose isomerase (AMF/PGI) and its receptor, the gp78/AMFR E3 ubiquitin ligase
Maria Fairbank, Pascal St-Pierre and Ivan R. Nabi,  Mol. BioSyst., 2009, 5, 793
DOI: 10.1039/b820820b
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b820820b)

Phosphoglucose isomerase (PGI) is a glycolytic enzyme and functions as an extracellular cytokine (autocrine motility factor, AMF). Its receptor is the E3 ubiquitin ligase gp78/AMFR. Expression of both AMF/PGI and gp78/AMFR is associated with cancer and various aspects of this ligand–receptor complex are discussed.

Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function
Daniel L. MinorJr,  Mol. BioSyst., 2009, 5, 802
DOI: 10.1039/b901708a
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b901708a)

Minor highlights the application of selection and screening methods to address basic questions about ion channels, ion channel–small molecule interactions, and the development of novel agents to control channel activity.

Application

Extracellular matrix and tissue engineering applications
Hugo Fernandes, Lorenzo Moroni, Clemens van Blitterswijk and Jan de Boer,  J. Mater. Chem., 2009, 19, 5474
DOI: 10.1039/b822177d

graphical abstract image (ID: b822177d)

Designing instructive materials able to mimic the features of extracellular matrix is of paramount importance to control cell–material interactions. Unraveling ECM complexity will lead to rational design of novel biomaterials.

Critical Reviews

Xanthosine 5-monophosphate (XMP). Acid–base and metal ion-binding properties of a chameleon-like nucleotide
Helmut Sigel, Bert P. Operschall and Rolf Griesser,  Chem. Soc. Rev., 2009, 38, 2465
DOI: 10.1039/b902181g

graphical abstract image (ID: b902181g)

XMP2- is commonly shown in textbooks analogous to guanosine 5-monophosphate (GMP2-), yet at the physiological pH it is actually present as (X-H·MP)3- and, of course, this affects its metal ion-binding properties.

Electrochemical biosensors at the nanoscale
Di Wei, Marc J. A. Bailey, Piers Andrew and Tapani Ryhänen,  Lab Chip, 2009, 9, 2123
DOI: 10.1039/b903118a

graphical abstract image (ID: b903118a)

A review of recent developments on the use of nanoscale structures in electrochemical sensors with a focus on their applications to biosensors.

Feature Articles

Advances in gene delivery through molecular design of cationic lipids
Santanu Bhattacharya and Avinash Bajaj,  Chem. Commun., 2009, 4632
DOI: 10.1039/b900666b

graphical abstract image (ID: b900666b)

This feature article discusses the structure–activity relation of various cationic liposomes for DNA/si-RNA/oligonucleotide delivery, the liposome and lipoplex characterizations, cytotoxicity and the biological applications of the lipid-based gene delivery vehicles.

Organic nanoscale drug carriers coupled with ligands for targeted drug delivery in cancer
Meng Shi, Jiao Lu and Molly S. Shoichet,  J. Mater. Chem., 2009, 19, 5485
DOI: 10.1039/b822319j

graphical abstract image (ID: b822319j)

We provide an updated, comprehensive review on organic nanocarriers with targeting ligands coupled on the surface, with a focus on surface conjugation chemistries that are used in nanoparticle systems. Targeted nanocarriers represent a new generation in anticancer therapy for delivery of combination immunotherapy/chemotherapy to more effectively treat certain malignancies.

Highlights

On the polyelectrolyte brush model of neurofilaments
E. B. Zhulina and F. A. M. Leermakers,  Soft Matter, 2009, 5, 2836
DOI: 10.1039/b903683k

graphical abstract image (ID: b903683k)

A polymer brush model for neurofilaments gives insight in how phosphorylation influences the three neurofilament proteins NF-L, NF-M and NF-H. First M and then H go from coil to flower conformation.

Nanoparticle technology: Addressing the fundamental roadblocks to protein biomarker discovery
Alessandra Luchini, Caterina Longo, Virginia Espina, Emanuel F. Petricoin III and Lance A. Liotta,  J. Mater. Chem., 2009, 19, 5071
DOI: 10.1039/b822264a

graphical abstract image (ID: b822264a)

In complex biologic fluids, core-shell, affinity-bait-containing, N-isopropylacrylamide-based hydrogel particles are able to: (a) sieve molecules according to size, (b) sequestrate and concentrate target analytes, and (c) protect analytes from degradation.

New natural product biosynthetic chemistry discovered by genome mining
Christophe Corre and Gregory L. Challis,  Nat. Prod. Rep., 2009, 26, 977
DOI: 10.1039/b713024b

graphical abstract image (ID: b713024b)

Genomics has led to the discovery of numerous new natural product biosynthetic enzymes. Many are involved in the assembly of novel natural products and some catalyse unprecedented transformations via fascinating and sometimes unanticipated mechanisms.

Structural insights into nonribosomal peptide enzymatic assembly lines
Alexander Koglin and Christopher T. Walsh,  Nat. Prod. Rep., 2009, 26, 987
DOI: 10.1039/b904543k

graphical abstract image (ID: b904543k)

Remarkable accomplishments have been made in the structure determination of FAS and PKS systems. This complete picture of NRPS assembly line structures is still not available. Recent progress on NRPS structures revealed details about domain orientations and protein–protein recognitions.

Hot off the Press Article

Hot off the press
Robert A. Hill and Andrew Sutherland,  Nat. Prod. Rep., 2009, 26, 973
DOI: 10.1039/b912059a

graphical abstract image (ID: b912059a)

A personal selection of 35 recent papers is presented covering various aspects of current developments in bioorganic chemistry and novel natural products such as the alkaloid–lignan conjugate conofoliferine from Tabernaemontana corymbosa.

Minireview

Labeling strategies in mass spectrometry-based protein quantitation
Randi L. Gant-Branum, Thomas J. Kerr and John A. McLean,  Analyst, 2009, 134, 1525
DOI: 10.1039/b904643g

graphical abstract image (ID: b904643g)

This article describes contemporary and emerging labeling strategies for mass spectrometry-based relative and absolute quantitation using natural and chemical modification stable isotope incorporation.

Perspective

Superparamagnetic nanoparticles as targeted probes for diagnostic and therapeutic applications
Chenjie Xu and Shouheng Sun,  Dalton Trans., 2009, 5583
DOI: 10.1039/b900272n

graphical abstract image (ID: b900272n)

With proper functionalization, magnetic nanoparticles can target specifically to cells. This Perspective discusses magnetic nanoparticle synthesis and surface functionalization for magnetic cellular imaging and drug delivery applications.

Technical Notes

Microfluidic enrichment of a target cell type from a heterogenous suspension by adhesion-based negative selection
James V. Green and Shashi K. Murthy,  Lab Chip, 2009, 9, 2245
DOI: 10.1039/b906768j

graphical abstract image (ID: b906768j)

Spiral geometry channels are utilized for enrichment of stem cells from a heterogeneous suspension.

Transport of live yeast and zebrafish embryo on a droplet (digital) microfluidic platform
Sang Uk Son and Robin L. Garrell,  Lab Chip, 2009, 9, 2398
DOI: 10.1039/b906257b

graphical abstract image (ID: b906257b)

This paper demonstrates programmed transport of eukaryotic organisms on a two-plate droplet microfluidic device. Yeast remain viable and zebrafish embryos hatch normally. On-chip dechorionation of the embryo is also shown. A new apparatus for continuously varying the gap between plates enables manipulation of droplets from the nL to µL scale.

An easy protocol for on-chip transfection of COS-7 cells with a cationic lipid-based reagent
Lei Li, Yong Nie, Datian Ye and Guoping Cai,  Lab Chip, 2009, 9, 2230
DOI: 10.1039/b901591d

graphical abstract image (ID: b901591d)

A microchip made of PDMS and glass was fabricated for continuous cell culture and transfection. This on-chip transfection protocol with cationic lipid-based reagent is expected to add to the emerging use of microchip-based life science and drug discovery research.

Dielectrophoresis-based cell manipulation using electrodes on a reusable printed circuit board
Kidong Park, Ho-Jun Suk, Demir Akin and Rashid Bashir,  Lab Chip, 2009, 9, 2224
DOI: 10.1039/b904328d

graphical abstract image (ID: b904328d)

In this study, a convenient and affordable approach to implement dielectrophoresis (DEP) particle manipulation without chip-based micro-fabrication is demonstrated.

Tutorial Review

Biological implications of polydimethylsiloxane-based microfluidic cell culture
Keil J. Regehr, Maribella Domenech, Justin T. Koepsel, Kristopher C. Carver, Stephanie J. Ellison-Zelski, William L. Murphy, Linda A. Schuler, Elaine T. Alarid and David J. Beebe,  Lab Chip, 2009, 9, 2132
DOI: 10.1039/b903043c

graphical abstract image (ID: b903043c)

Uncured PDMS leaches into microchannel media, and estrogen partitions out. These material properties may complicate cell culture data in microchannels.

Communications

Substrate and reaction intermediate mimics as inhibitors of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
Scott R. Walker, Hemi Cumming and Emily J. Parker,  Org. Biomol. Chem., 2009, 7, 3031
DOI: 10.1039/b909241b

graphical abstract image (ID: b909241b)

A series of compounds designed to mimic the properties of substrate phosphoenolpyruvate and the predicted reaction intermediate were synthesised and shown to be effective inhibitors of 3-deoxy-D-arabino-heptulosonate phosphate (DAH7P) synthase, the first enzyme of the shikimate pathway.

Targeting lectin activity into inclusion bodies for the characterisation of glycoproteins
Jozef Nahálka, Danica Misloviová and Helena Kavcová,  Mol. BioSyst., 2009, 5, 819
DOI: 10.1039/b900526a
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b900526a)

Lectin activity can be deliberately targeted into inclusion bodies. Since proteins expressed as inclusion bodies are especially easy to purify, a simple IB-agglutination method to produce an array of glycoproteins is proposed.

Synthesis and cellular uptake properties of guanidine-containing molecular transporters built on the sucrose scaffold
Woo Sirl Lee, Chang-Nim Im, Qi Ying Teng, Young-Tae Chang, Dong-Chan Kim, Kyong-Tae Kim and Sung-Kee Chung,  Mol. BioSyst., 2009, 5, 822
DOI: 10.1039/b901846h
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b901846h)

Novel sucrose-based G7 molecular transporters show different patterns of intracellular localization depending on the nature of the linker chains as well as the fluorescent dyes.

Discovery of a new PCC-mediated stereoselective oxidative spiroketalization process. An access to a new type of poly-THF spiroketal compound displaying anticancer activity
Vincenzo Piccialli, Giorgia Oliviero, Nicola Borbone, Angela Tuzi, Roberto Centore, Akseli Hemminki, Matteo Ugolini and Vincenzo Cerullo,  Org. Biomol. Chem., 2009, 7, 3036
DOI: 10.1039/b906872d

graphical abstract image (ID: b906872d)

A new type of cytotoxic poly-THF compound, embodying a novel tricyclic spiroketal moiety, has been obtained through an unprecedented PCC-mediated stereoselective oxidative spiroketalization process.

Structure–energy relations in hen egg white lysozyme observed during refolding from a quenched unfolded state
Theresa Y. Cho, Nolene Byrne, David J. Moore, Brian A. Pethica, C. Austen Angell and Pablo G. Debenedetti,  Chem. Commun., 2009, 4441
DOI: 10.1039/b907656e

graphical abstract image (ID: b907656e)

Low-temperature structural changes that precede the formation of energetic native contacts in hen egg white lysozyme are revealed using a quench-and-refold approach and infrared spectroscopy.

Hydrophilic multi-walled carbon nanotubes decorated with magnetite nanoparticles as lymphatic targeted drug delivery vehicles
Dong Yang, Feng Yang, Jianhua Hu, Jiang Long, Changchun Wang, Deliang Fu and Quanxing Ni,  Chem. Commun., 2009, 4447
DOI: 10.1039/b908012k
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b908012k)

Hydrophilic multi-walled carbon nanotubes decorated with magnetite nanoparticles were readily taken up into lymph vessels and delivered gemcitabine to lymph nodes with high efficiency under the guidance of a magnetic field.

Magneto-optical FeGa2O4 nanoparticles as dual-modality high contrast efficacy T2 imaging and cathodoluminescent agents
Chih-Chia Huang, Chia-Hao Su, Mei-Yi Liao and Chen-Sheng Yeh,  Phys. Chem. Chem. Phys., 2009, 11, 6331
DOI: 10.1039/b907782k

graphical abstract image (ID: b907782k)

Spinel-structured magneto-luminescent FeGa2O4 nanoparticles (25 nm) were prepared and exhibited high Fe mass magnetization with a high-performance T2-lowering effect and cathodoluminescence.

An ethylene-glycol decorated ruthenium(II) complex for two-photon photodynamic therapy
Sanda C. Boca, Mickaël Four, Adeline Bonne, Boudewijn van der Sanden, Simion Astilean, Patrice L. Baldeck and Gilles Lemercier,  Chem. Commun., 2009, 4590
DOI: 10.1039/b907143a
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b907143a)

A novel water-soluble tris(fluorene-substituted-1,10-phenanthroline) Ru(II) complex has been characterized as a promising new class of selective coordination photosensitizer able to generate 1O2 in a confined space.

A DNA-binding Gd chelate for the detection of cell death by MRI
Elisabeth Garanger, Scott A. Hilderbrand, Joseph T. Blois, David E. Sosnovik, Ralph Weissleder and Lee Josephson,  Chem. Commun., 2009, 4444
DOI: 10.1039/b907375b

graphical abstract image (ID: b907375b)

GadoTO is a Gd bearing vital fluorochrome that selectively traversed the compromised membranes of dead cells and bound DNA. Uptake by dead cells was evident by microscopy, flow cytometry and a decreased cellular T1.

A cryptand based chemodosimetric probe for naked-eye detection of mercury(II) ion in aqueous medium and its application in live cell imaging
Atanu Jana, Jong Seung Kim, Hyo Sung Jung and Parimal K. Bharadwaj,  Chem. Commun., 2009, 4417
DOI: 10.1039/b907646h

graphical abstract image (ID: b907646h)

A novel chemodosimeter with high quantum yield ( = 0.51) can detect Hg(II) ion selectively at ppb level in aqueous medium and also effectively shows a good viability to HEK 293 cell line.

Preparation of a 3-azido-3-deoxythymidine (AZT) derivative, which is blood–brain barrier permeable
Jungkyun Im, Wanil Kim, Kyong-Tai Kim and Sung-Kee Chung,  Chem. Commun., 2009, 4669
DOI: 10.1039/b909541a

graphical abstract image (ID: b909541a)

The anti-AIDS drug AZT was covalently attached to the recently reported sorbitol-G8 transporter, and the conjugate was found to target mitochondria in HeLa cells and readily cross the BBB to gain access into the mouse brain, suggesting its potential as a practical CNS delivery vector.

Solid-phase synthesis and DNA binding studies of dichloroplatinum(II) conjugates of dicarba analogues of octreotide as new anticancer drugs
Flavia Barragán, Virtudes Moreno and Vicente Marchán,  Chem. Commun., 2009, 4705
DOI: 10.1039/b909698a

graphical abstract image (ID: b909698a)

The first dichloroplatinum(II) conjugates of dicarba analogues of octreotide, which is expected to act as a tumour-targeting device, have been efficiently synthesized following a stepwise solid-phase approach. These compounds emulate the mechanism of cisplatin since they form a 1,2-intrastrand cross-link with two consecutive guanines of a DNA chain.

Tyrosine-67 in cytochrome c is a possible apoptotic trigger controlled by hydrogen bonds via a conformational transition
Tianlei Ying, Zhong-Hua Wang, Ying-Wu Lin, Jin Xie, Xiangshi Tan and Zhong-Xian Huang,  Chem. Commun., 2009, 4512
DOI: 10.1039/b904347k
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b904347k)

Cyt c Y67H and Y67R represent a state which resembles the conformational intermediate state in cyt c with high peroxidase activity; the hydrogen bond network around Tyr67 is essential in maintaining the cyt c functioning not only as an ET protein but also probably as a trigger in apoptosis.

A simple approach for the discrimination of nucleotides based on a water-soluble polythiophene derivative
Zhiyi Yao, Xueling Feng, Wenjing Hong, Chun Li and Gaoquan Shi,  Chem. Commun., 2009, 4696
DOI: 10.1039/b904975d

graphical abstract image (ID: b904975d)

A sensor based on an array of wavelengths of a cationic poythiophene derivative was applied to discriminate fifteen nucleotides along with monophosphate and pyrophosphate with 100% confidence limits.

Label-free electrochemical detection of adenosine based on electron transfer from guanine bases in an adenosine-sensitive aptamer
Jinheung Kim, In Young Kim, Min Sun Choi and Qiong Wu,  Chem. Commun., 2009, 4747
DOI: 10.1039/b908344h

graphical abstract image (ID: b908344h)

We report a label-free electrochemical method for the detection of adenosine using electron transfer between the guanine bases of a single-stranded oligonucleotide probe and Ru(bpy)33+.

Fluorescent probe for detection of fluoride in water and bioimaging in A549 human lung carcinoma cells
Soon Young Kim, Jongmin Park, Minseob Koh, Seung Bum Park and Jong-In Hong,  Chem. Commun., 2009, 4735
DOI: 10.1039/b908745a

graphical abstract image (ID: b908745a)

We successfully developed TBPCA as a fluoride ion probe for fluorescence cell bioimaging with desired properties, such as the detaining of the fluorophore inside cells, non-cytotoxicity to mammalian cells, fluorescence upon sensing, appreciable solubility in water, and stoichiometric reaction with analytes.

Nanopore DNA sensors based on dendrimer-modified nanopipettes
Yaqin Fu, Hideo Tokuhisa and Lane A. Baker,  Chem. Commun., 2009, 4877
DOI: 10.1039/b910511e
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b910511e)

A dendrimer-modified nanopipette is used to detect hybridization of a specific DNA sequence through evaluation of the extent of rectification of ion currents observed in the measured current–voltage response.

Folding-based electrochemical DNA sensor fabricated by click chemistry
Socrates Jose P. Cañete, Weiwei Yang and Rebecca Y. Lai,  Chem. Commun., 2009, 4835
DOI: 10.1039/b911273a
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b911273a)

Here we report a folding-based electrochemical DNA (E-DNA) sensor fabricated by Sharpless click chemistry. The click chemistry-based E-DNA sensor compares favorably to a sensor fabricated via the conventional two-step approach.

Multicomponent reactions for the synthesis of multifunctional agents with activity against cancer cells
Saad Shabaan, Lalla A. Ba, Muhammad Abbas, Torsten Burkholz, Annika Denkert, André Gohr, Ludger A. Wessjohann, Florenz Sasse, Wolfgang Weber and Claus Jacob,  Chem. Commun., 2009, 4702
DOI: 10.1039/b823149d
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b823149d)

Multicomponent reactions enable the fast and efficient synthesis of multifunctional compounds with cytotoxicity against specific cancer cells.

Facile analysis of metabolites by capillary electrophoresis coupled to matrix-assisted laser desorption/ionization mass spectrometry using target plates with polysilazane nanocoating and grooves
Andrea Amantonico, Pawel L. Urban and Renato Zenobi,  Analyst, 2009, 134, 1536
DOI: 10.1039/b907039g

graphical abstract image (ID: b907039g)

We present an inexpensive method and apparatus for the deposition of analytes separated by CE onto a custom-designed MALDI target. This straightforward protocol was applied to the analysis of metabolites from the central metabolic pathway in yeast.

Antimicrobial gold nanorods with dual-modality photodynamic inactivation and hyperthermia
Wen-Shuo Kuo, Chich-Neng Chang, Yi-Ting Chang and Chen-Sheng Yeh,  Chem. Commun., 2009, 4853
DOI: 10.1039/b907274h

graphical abstract image (ID: b907274h)

Gold nanorods coated with a PAA polymer and TBO (Au-PAA-TBO nanorods) served as photodynamic antimicrobial chemotherapy and hyperthermia agents to destroy multidrug resistant bacteria.

Bifunctional bisphosphonate complexes for the diagnosis and therapy of bone metastases
R. Torres Martin de Rosales, C. Finucane, S. J. Mather and P. J. Blower,  Chem. Commun., 2009, 4847
DOI: 10.1039/b908652h
RSC Prospect Icon Enhanced HTML article available

graphical abstract image (ID: b908652h)

Easily synthesised and structurally well-defined novel imaging and therapeutic radiopharmaceuticals with potential as agents for bone metastases are described.

Papers

Polyelectrolyte–protein complexation driven by charge regulation
Fernando Luís Barroso da Silva and Bo Jönsson,  Soft Matter, 2009, 5, 2862
DOI: 10.1039/b902039j

graphical abstract image (ID: b902039j)

Model system containing a titrating protein and a polyelectrolyte chain plus salt in a cell model.

Comparison of the PLA-mPEG and mPEG-PLA-mPEG copolymers nanoparticles on the plasma protein adsorption and in vivo biodistribution
Xiaoqian Shan, Yuan Yuan, Changsheng Liu, Feng Xu and Yan Sheng,  Soft Matter, 2009, 5, 2875
DOI: 10.1039/b902714a

graphical abstract image (ID: b902714a)

A protein adsorption study with models of fibrinogen and BSA onto these polymer surfaces was performed. The control PLA showed the largest values of protein adsorption which resulted in uptake by phagocytosis rapidly.

Plasmon-induced photothermal cell-killing effect of gold colloidal nanoparticles on epithelial carcinoma cells
Md. Abdulla-Al-Mamun, Yoshihumi Kusumoto, Aki Mihata, Md. Shariful Islam and Bashir Ahmmad,  Photochem. Photobiol. Sci., 2009, 8, 1125
DOI: 10.1039/b907524k

graphical abstract image (ID: b907524k)

Plasmon-induced gold colloidal nanoparticles absorb visible light and then photoenergy converted to local heat energy on and/or in cancer cells is used for photothermal cancer cell killing therapy.

Site-specific N-terminus conjugation of poly(mPEG1100) methacrylates to salmon calcitonin: synthesis and preliminary biological evaluation
Claire T. Sayers, Giuseppe Mantovani, Sinead M. Ryan, Rajan K. Randev, Odin Keiper, Oksana I. Leszczyszyn, Claudia Blindauer, David J. Brayden and David M. Haddleton,  Soft Matter, 2009, 5, 3038
DOI: 10.1039/b905335b

graphical abstract image (ID: b905335b)

Comb shaped polymers have been conjugated to salmon calcitonin at the N-terminus with a view to improving the efficacy of this osteoporosis drug.

Power spectrum of out-of-equilibrium forces in living cells: amplitude and frequency dependence
François Gallet, Delphine Arcizet, Pierre Bohec and Alain Richert,  Soft Matter, 2009, 5, 2947
DOI: 10.1039/b901311c

graphical abstract image (ID: b901311c)

By combining simultaneous active and passive microrheology measurements in living cells, we infer the power spectrum of active forces generated in the out-of-equilibrium intracellular medium

DNA sequence-dependent enhancement of Cy3 fluorescence
Billie Jo Harvey, Claudia Perez and Marcia Levitus,  Photochem. Photobiol. Sci., 2009, 8, 1105
DOI: 10.1039/b905078g

graphical abstract image (ID: b905078g)

The photophysical properties of Cy3 covalently attached to the 5 terminus of DNA were found to be very sensitive to the sequence and flexibility of the oligonucleotide. The fluorescence efficiency of the dye increases considerably in those cases where Cy3 closely interacts with the DNA bases.

Computational study of khellin excited states and photobinding to DNA
Salama Omar and Leif A. Eriksson,  Photochem. Photobiol. Sci., 2009, 8, 1179
DOI: 10.1039/b905147c

graphical abstract image (ID: b905147c)

The photoinduced binding between khellin and nucleobase thymine is studied using computational quantum chemistry.

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Alessandra Andreoni, Maria Bondani and Luca Nardo,  Photochem. Photobiol. Sci., 2009, 8, 1202
DOI: 10.1039/b906043j

graphical abstract image (ID: b906043j)

Among the HLA-DQB1 antigens, we identify the 0201 variant, most frequently found in patients affected by insulin-dependent diabetes mellitus in an Italian region, by demonstrating that annealing to 0201 brings the donor and acceptor labeling our probe at greater distance than annealing to any other variant.

Total syntheses of coronatines by exo-selective Diels–Alder reaction and their biological activities on stomatal opening
Masahiro Okada, Satoko Ito, Akira Matsubara, Izumi Iwakura, Syusuke Egoshi and Minoru Ueda,  Org. Biomol. Chem., 2009, 7, 3065
DOI: 10.1039/b905159g

graphical abstract image (ID: b905159g)

We synthesized four stereoisomers of coronatine employing the exo-selective Diels–Alder reaction as a key step and examined the effect of stereochemistry on stomatal opening activity.

Topical photodynamic therapy with porphyrin precursors—assessment of treatment-associated pain in a retrospective study
Julia Maria Steinbauer, Stephan Schreml, Philipp Babilas, Florian Zeman, Sigrid Karrer, Michael Landthaler and Rolf-Markus Szeimies,  Photochem. Photobiol. Sci., 2009, 8, 1111
DOI: 10.1039/b823378k

graphical abstract image (ID: b823378k)

Predictor variables for higher treatment-associated pain in PDT are the photosensitizer used (ALA), the lesion type (AK) and localization (head).

Measurements of the anatomical distribution of erythemal ultraviolet: a study comparing exposure distribution to the site incidence of solar keratoses, basal cell carcinoma and squamous cell carcinoma
Nathan Downs and Alfio Parisi,  Photochem. Photobiol. Sci., 2009, 8, 1195
DOI: 10.1039/b901741k

graphical abstract image (ID: b901741k)

High density measurements of solar ultraviolet (UV) exposure were made using miniaturized polysulfone dosimeters attached to the face, neck, arm, hand and leg of a life size model of an adult human. These data were compared to published body site incidence data for solar keratoses, basal and squamous cell carcinoma.

Synthesis and biological evaluation of functionalised tetrahydro--carboline analogues as inhibitors of Toxoplasma gondii invasion
Jeffrey G. A. Walton, Stephen Patterson, Gu Liu, Jeralyn D. Haraldsen, Jonathan J. Hollick, Alexandra M. Z. Slawin, Gary E. Ward and Nicholas J. Westwood,  Org. Biomol. Chem., 2009, 7, 3049
DOI: 10.1039/b902319d

graphical abstract image (ID: b902319d)

The synthesis of two CIDs for use in the yeast-3-hybrid approach to protein target identification is described. Both CIDs are based on the screening hit shown.

Display Records 0 to 60 of 125 records