Issue 6, 2003
From the journal:

Chemical Communications

First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination†

T. P. Nicholson,a   C. Winfield,a   J. Westcott,a   J. Crosby,a   T. J. Simpsona  and  R. J. Cox*a  

* Corresponding authors

a School of Chemistry, University of Bristol, Cantock′s Close, Bristol, UK
E-mail: r.j.cox@bris.ac.uk

Abstract

The minimal actinorhodin polyketide synthase bearing two point mutations (KSβ Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism.

Graphical abstract: First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

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Article information

DOI
https://doi.org/10.1039/B300847A
Article type
Communication
Submitted
22 Jan 2003
Accepted
06 Feb 2003
First published
19 Feb 2003

Chem. Commun., 2003, 686-687

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First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination

T. P. Nicholson, C. Winfield, J. Westcott, J. Crosby, T. J. Simpson and R. J. Cox, Chem. Commun., 2003, 686 DOI: 10.1039/B300847A

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