Issue 15, 2005
From the journal:

Physical Chemistry Chemical Physics

The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules†

Liming Ying,a   Andreas Bruckbauer,a   Dejian Zhou,a   Julia Gorelik,b   Andrew Shevchuk,b   Max Lab,b   Yuri Korchevb  and  David Klenerman*a  

* Corresponding authors

a Department of Chemistry, Cambridge University, Lensfield Road, Cambridge, UK
E-mail: dk10012@cam.ac.uk

b Division of Medicine, Imperial College School of Medicine, MRC Clinical Sciences Centre, London, UK

Abstract

The boundary between the physical and biological sciences has been eroded in recent years with new physical methods applied to biology and biological molecules being used for new physical purposes. We have pioneered the application of a form of scanning probe microscopy based on a scanned nanopipette, originally developed by Hansma and co-workers, for reliable non-contact imaging over the surface of a live cell. We have found that the nanopipette can also be used for controlled local voltage-driven application of reagents or biomolecules and this can be used for controlled deposition and the local delivery of probes for mapping of specific species. In this article we review this progress, focussing on the physical principles and new phenomena that we have observed, and then outline the future applications that are now possible.

Graphical abstract: The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules

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Article information

DOI
https://doi.org/10.1039/B506743J
Article type
Invited Article
Submitted
12 May 2005
Accepted
16 Jun 2005
First published
05 Jul 2005

Phys. Chem. Chem. Phys., 2005,7, 2859-2866

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The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules

L. Ying, A. Bruckbauer, D. Zhou, J. Gorelik, A. Shevchuk, M. Lab, Y. Korchev and D. Klenerman, Phys. Chem. Chem. Phys., 2005, 7, 2859 DOI: 10.1039/B506743J

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