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Paper
Phys. Chem. Chem. Phys., 2008, 10, 6741 - 6750, DOI: 10.1039/b808938h
About the albumin structure in solution: cigar Expanded form versus heart Normal shape
Claudia Leggio, Luciano Galantini and Nicolae Viorel Pavel
A structural comparison between the Normal and the Expanded isomers of the human serum albumin has been carried out by using small angle X-ray scattering (SAXS) and light scattering (LS) techniques. Geometrical bodies, recovered structures (GA_STRUCT code) and rigid body modeling (CRYSOL and BUNCH software) were used to obtain low-resolution 3D structures from one-dimensional scattering patterns. These restored shapes were also exploited to perform a correlation between SAXS and LS data. By attempting a detailed description of globular and unfolded protein structures in solution, we tried to propose a suitable approach to follow the path of folding/unfolding processes and to isolate and characterize possible partially folded intermediate states.
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