Issue 14, 2010
From the journal:

Physical Chemistry Chemical Physics

Peptide fragmentation by keV ion-induced dissociation

Sadia Bari,*a   Ronnie Hoekstraa  and  Thomas Schlathöltera  

* Corresponding authors

a KVI Atomic and Molecular Physics, University of Groningen, Zernikelaan 25, NL-9747 AA Groningen, The Netherlands
E-mail: s.bari@rug.nl

Abstract

We have studied multiple ionization and dissociation of a trapped protonated peptide (leucine enkephalin) as induced by keV singly and doubly charged ions (H+, He+, 2+) to demonstrate the potential of keV ions as a future tool for peptide identification. In contrast to conventional excitation techniques, the fragmentation pattern exhibits very strong peaks due to loss of sidechains in addition to those due to backbone scission. The results can be understood on the basis of the energy deposited into the peptide via electronic stopping. A pronounced dependence of the fragmentation pattern on the electronic structure of the projectile ions can be attributed to different electron capture efficiencies from localized molecular orbitals.

Graphical abstract: Peptide fragmentation by keV ion-induced dissociation

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Article information

DOI
https://doi.org/10.1039/B924145K
Article type
Paper
Submitted
17 Nov 2009
Accepted
01 Feb 2010
First published
24 Feb 2010

Phys. Chem. Chem. Phys., 2010,12, 3376-3383

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Peptide fragmentation by keV ion-induced dissociation

S. Bari, R. Hoekstra and T. Schlathölter, Phys. Chem. Chem. Phys., 2010, 12, 3376 DOI: 10.1039/B924145K

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