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Technical Note

J. Anal. At. Spectrom., 2007, 22, 1193 - 1198, DOI: 10.1039/b704577h

Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

Marco Malavolta, Francesco Piacenza, Laura Costarelli, Robertina Giacconi, Elisa Muti, Catia Cipriano, Silvia Tesei, Sandro Spezia and Eugenio Mocchegiani

A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount.

Graphical abstract image for this article (ID: b704577h)