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Latest News
Spy moths controlled by chemicals
26 November 2008
US researchers control moth flight using microfluidic implants
Counting the drops
21 November 2008
Korean scientists have created a microfluidic abacus that allows them to add droplets together
IUPAC 2009 - Call for Abstracts
21 November 2008
Your chance to take part in 42nd IUPAC Congress (2-7 August 2009, Glasgow, UK). Oral abstract deadline - 16 January 2009
Lab on a Chip Themed Issue: Point-of-Care Diagnostics
19 November 2008
This special issue captures some of the leading directions and themes of today's research.
Contents list for Lab on a Chip, issue 12, 2008
Front cover
Lab Chip, 2008, 8, 1965
DOI: 10.1039/b819445a
Contents and Chemical Technology
Lab Chip, 2008, 8, 1967
DOI: 10.1039/b819447p
Highlight
Research Highlights
Lab Chip, 2008, 8, 1979
DOI: 10.1039/b818374k
Petra Dittrich reviews the current literature in miniaturisation and related technologies.
Editorial
Microfluidics and point-of-care testing
Samuel K. Sia and Larry J. Kricka,
Lab Chip, 2008, 8, 1982
DOI: 10.1039/b817915h
Samuel Sia and Larry Kricka introduce the articles featured in the themed issue on Point-of-care Microfluidic Diagnostics covering a range of topics in microfluidics and POC testing.
Profile
Main contributors to the Point-of-care Microfluidic Diagnostics issue
Lab Chip, 2008, 8, 1984
DOI: 10.1039/b818692h
Biographical profiles and photographs of the main contributors to the themed issue on Point-of-care Microfluidic Diagnostics.
Focus
Lab on paper
Lab Chip, 2008, 8, 1988
DOI: 10.1039/b814043j
Inexpensive and easy-to-use paper-based bioassay represents one practically useful platform for point-of-care diagnostics, particularly for those in developing countries.
Perspective
Microchip-based cell analysis and clinical diagnosis system
Kae Sato, Kazuma Mawatari and Takehiko Kitamori,
Lab Chip, 2008, 8, 1992
DOI: 10.1039/b814098g
Cell analysis and clinical diagnosis systems are now becoming the largest field of application for microchip-based analytical systems. Technological advantages include: small volume, fast analysis time, highly integrated analytical functions, easy operation and small size.
Critical Reviews
Towards non- and minimally instrumented, microfluidics-based diagnostic devices
Bernhard Weigl, Gonzalo Domingo, Paul LaBarre and Jay Gerlach,
Lab Chip, 2008, 8, 1999
DOI: 10.1039/b811314a
Preliminary design of a non-instrumented NAAT assay for malaria based on an exothermically heated LAMP assay.
Innovations in optical microfluidic technologies for point-of-care diagnostics
Frank B. Myers and Luke P. Lee,
Lab Chip, 2008, 8, 2015
DOI: 10.1039/b812343h
In this review, we highlight recent advancements in the integration of optical detection systems and microfluidics for point-of-care diagnostics. Both on-chip and off-chip integration strategies are examined, along with emerging nanoscale optical detection technologies.
Papers
Egg beater as centrifuge: isolating human blood plasma from whole blood in resource-poor settings
Amy P. Wong, Malancha Gupta, Sergey S. Shevkoplyas and George M. Whitesides,
Lab Chip, 2008, 8, 2032
DOI: 10.1039/b809830c
A hand-held egg beater, combined with flexible polyethylene tubing, can serve as a centrifuge for obtaining plasma from whole blood in resource-poor settings.
Enabling a microfluidic immunoassay for the developing world by integration of on-card dry reagent storage
Dean Y. Stevens, Camille R. Petri, Jennifer L. Osborn, Paolo Spicar-Mihalic, Katherine G. McKenzie and Paul Yager,
Lab Chip, 2008, 8, 2038
DOI: 10.1039/b811158h
A method for incorporating dry-reagent storage in microfluidic assays is demonstrated and characterized, enabling development of a flow-through membrane immunoassay designed for point-of-care detection of malaria in the developing world.
An integrated microfluidic platform for sensitive and rapid detection of biological toxins
Robert J. Meagher, Anson V. Hatch, Ronald F. Renzi and Anup K. Singh,
Lab Chip, 2008, 8, 2046
DOI: 10.1039/b815152k
We have designed a portable device for potential application in point-of-care diagnosis of biotoxin exposure. We demonstrate microfluidic immunoassays for ricin, Shiga toxin 1, and Staphylococcal enterotoxin B, with detection limits <10 pM enabled by integrated sample preconcentration.
Neutrophil migration assay from a drop of blood
Nitin Agrawal, Mehmet Toner and Daniel Irimia,
Lab Chip, 2008, 8, 2054
DOI: 10.1039/b813588f
We have developed a straightforward in vitro chemotaxis assay that uses neutrophils isolated directly from a drop of blood and closely mimics in vivo scenarios.
Effect of volume- and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays
Hesam Parsa, Curtis D. Chin, Puttisarn Mongkolwisetwara, Benjamin W. Lee, Jennifer J. Wang and Samuel K. Sia,
Lab Chip, 2008, 8, 2062
DOI: 10.1039/b813350f
This study presents computational results and corresponding experimental binding assays in which the capture of analytes is studied under variations in both mass transfer and surface binding, constrained by real-world assay conditions of finite sample volume, assay time, and capture area.
Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics
Ivan K. Dimov, Jose L. Garcia-Cordero, Justin O'Grady, Claus R. Poulsen, Caroline Viguier, Lorcan Kent, Paul Daly, Bryan Lincoln, Majella Maher, Richard O'Kennedy, Terry J. Smith, Antonio J. Ricco and Luke P. Lee,
Lab Chip, 2008, 8, 2071
DOI: 10.1039/b812515e
We demonstrate the first microfluidic integration of RNA-purification and real-time NASBA.
Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need
Jesse V. Jokerst, Pierre N. Floriano, Nicolaos Christodoulides, Glennon W. Simmons and John T. McDevitt,
Lab Chip, 2008, 8, 2079
DOI: 10.1039/b817116e
As part of continuing efforts to develop practical diagnostic instrumentation, the integration of semiconductor nanocrystals (quantum dots, QDs) into a portable microfluidic-based lymphocyte capture and detection device is completed here for measurement of CD4 cells for HIV immune function for use in both point-of-need and resource-scarce settings.
Development of a digital microfluidic platform for point of care testing
Ramakrishna Sista, Zhishan Hua, Prasanna Thwar, Arjun Sudarsan, Vijay Srinivasan, Allen Eckhardt, Michael Pollack and Vamsee Pamula,
Lab Chip, 2008, 8, 2091
DOI: 10.1039/b814922d
A digital microfluidic platform for point-of-care applications is presented with a focus on critical issues in the implementation of immunoassays and DNA amplification and the respective sample preparation methods.
Photolithographic patterning of organosilane monolayer for generating large area two-dimensional B lymphocyte arrays
Nan Li and Chih-Ming Ho,
Lab Chip, 2008, 8, 2105
DOI: 10.1039/b810329a
The hexamethyldisilazane (HMDS) and poly(ethylene glycol) (PEG) molecular binary surface is developed for generating high fidelity cell arrays. The antibodies immobilized through biotin–streptavidin complexation are highly active for immunocapturing cells.
Development of functional lab-on-a-chip on polymer for point-of-care testing of metabolic parameters
Jaephil Do, Sehwan Lee, Jungyup Han, Junhai Kai, Chien-Chong Hong, Chuan Gao, Joseph H. Nevin, Gregory Beaucage and Chong H. Ahn,
Lab Chip, 2008, 8, 2113
DOI: 10.1039/b811169c
This paper presents the development of an easy-to-handle and disposable clinical diagnostic lab-on-a-chip using fully integrated plastic microfluidic components, which has the sampling/identifying capability to make fast and reliable measurements of metabolic parameters from human whole blood.
A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics
Soo Hyun Lee, Sung-Woo Kim, Ji Yoon Kang and Chong H. Ahn,
Lab Chip, 2008, 8, 2121
DOI: 10.1039/b811131f
A fully disposable RT-PCR lab-on-a-chip with embedded micro pinch valves has been designed and fabricated on polymer substrates and fully characterized for RT-PCR procedures. In addition, a portable analyzer interfaced with the polymer LOC for RT-PCR has been developed and applied for the point-of-care testing clinical diagnostic of HIV using a chemiluminescence assay.
Microvortex for focusing, guiding and sorting of particles
Chia-Hsien Hsu, Dino Di Carlo, Chihchen Chen, Daniel Irimia and Mehmet Toner,
Lab Chip, 2008, 8, 2128
DOI: 10.1039/b813434k
We report a continuous particle manipulation method that utilizes passively generated microvortices for differential focusing and sorting of particulate samples in microchannels.
Microchip DNA electrophoresis with automated whole-gel scanning detection
Roger C. Lo and Victor M. Ugaz,
Lab Chip, 2008, 8, 2135
DOI: 10.1039/b811033f
We present an automated whole-gel scanning detection system that enables the progress of microchip-based gel electrophoresis of DNA to be continuously monitored along an entire microchannel for improved separation performance.
Technical Notes
FLASH: A rapid method for prototyping paper-based microfluidic devices
Andres W. Martinez, Scott T. Phillips, Benjamin J. Wiley, Malancha Gupta and George M. Whitesides,
Lab Chip, 2008, 8, 2146
DOI: 10.1039/b811135a
Paper-based microfluidic devices are emerging as a new technology for applications in diagnostics for the developing world, where low cost and simplicity are essential. This article describes FLASH, a rapid method for laboratory prototyping of microfluidic devices in paper.
An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization
Vincent J. Sieben, Carina S. Debes-Marun, Linda M. Pilarski and Christopher J. Backhouse,
Lab Chip, 2008, 8, 2151
DOI: 10.1039/b812443d
The microfluidic chip here performs FISH (a cell-by-cell genetic test) with minimal human intervention. The cells are from a male patient sample and have one X (green dot) and one Y (red dot) chromosome per cell (blue).
Communications
Photoreactive coating for high-contrast spatial patterning of microfluidic device wettability
Adam R. Abate, Amber T. Krummel, Daeyeon Lee, Manuel Marquez, Christian Holtze and David A. Weitz,
Lab Chip, 2008, 8, 2157
DOI: 10.1039/b813405g
For many applications in microfluidics, the wettability of the devices must be spatially controlled. We introduce a photoreactive sol–gel coating that enables high-contrast spatial patterning of microfluidic device wettability.
Surface patterning of (bio)molecules onto the inner wall of fused-silica capillary tubes
Nabil Dendane, Antoine Hoang, Olivier Renaudet, Françoise Vinet, Pascal Dumy and Eric Defrancq,
Lab Chip, 2008, 8, 2161
DOI: 10.1039/b811786a
An efficient photochemical method for the site-specific immobilization and patterning of (bio)molecules inside glass capillary tubes is reported. The strategy involves the photodeprotection of reactive aminooxy groups on surfaces and subsequent reaction with aldehyde containing (bio)molecules.
Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP
Jonathan M. Bélisle, James P. Correia, Paul W. Wiseman, Timothy E. Kennedy and Santiago Costantino,
Lab Chip, 2008, 8, 2164
DOI: 10.1039/b813897d
LAPAP is a laser-based method for generating substrate-bound protein gradients with micron resolution which has the simplicity to be implemented in life science laboratories.
Papers
Sequential enzymatic glycosyltransfer reactions on a microfluidic device: Synthesis of a glycosaminoglycan linkage region tetrasaccharide
Yasunari Ono, Motohiro Kitajima, Shusaku Daikoku, Toshifumi Shiroya, Shoko Nishihara, Yoshimi Kanie, Katsuhiko Suzuki, Satoshi Goto and Osamu Kanie,
Lab Chip, 2008, 8, 2168
DOI: 10.1039/b809316d
A sequential enzymatic reaction was achieved on a microfluidic system. The chip-based glycan synthesis might be used to investigate Golgi apparatus function.
Live cell lithography: Using optical tweezers to create synthetic tissue
Utkur Mirsaidov, Jan Scrimgeour, Winston Timp, Kaethe Beck, Mustafa Mir, Paul Matsudaira and Gregory Timp,
Lab Chip, 2008, 8, 2174
DOI: 10.1039/b807987k
Using optical tweezers and a step-and-repeat method, we can create synthetic tissue that has the potential to capture the three-dimensional complexity of a multi-cellular organism with submicron precision.
Microfluidic polymer multilayer adsorption on liquid crystal droplets for microcapsule synthesis
Craig Priest, Anthony Quinn, Almar Postma, Alexander N. Zelikin, John Ralston and Frank Caruso,
Lab Chip, 2008, 8, 2182
DOI: 10.1039/b808826h
Multistep adsorption of polymer on liquid crystal droplets for the synthesis of polymer microcapsules under continuous flow conditions. Droplet templates are generated in a continuous phase that can be selectively withdrawn and replaced by rinse and polymer solutions. These steps can be repeated in a single microchip to adsorb polymer multilayers on the templates, shown in the fluorescence micrograph.
Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform
Ramakrishna S. Sista, Allen E. Eckhardt, Vijay Srinivasan, Michael G. Pollack, Srinivas Palanki and Vamsee K. Pamula,
Lab Chip, 2008, 8, 2188
DOI: 10.1039/b807855f
Heterogeneous magnetic bead-based sandwich immunoassays were demonstrated on human insulin and interleukin-6 on a digital microfluidic platform.
A microdevice for multiplexed detection of T-cell-secreted cytokines
He Zhu, Gulnaz Stybayeva, Monica Macal, Erlan Ramanculov, Michael D. George, Satya Dandekar and Alexander Revzin,
Lab Chip, 2008, 8, 2197
DOI: 10.1039/b810244a
Printed microarrays of cell- and cytokine-specific antibodies were enclosed inside a microfluidic device, enabling capture of T-cells from human blood and on-chip detection of two types of secreted cytokines (IL-2 and IFN-gamma).
Tissue reconstruction in 3D-spheroids from rodent retina in a motion-free, bioreactor-based microstructure
Matthias Rieke, Eric Gottwald, Karl-Friedrich Weibezahn and Paul Gottlob Layer,
Lab Chip, 2008, 8, 2206
DOI: 10.1039/b806988c
We present the application of a complex bioreactor system with a micro-structured platform for the reconstruction of three-dimensional retinal tissues. This is a key component for future high-throughput investigations in environmental monitoring and biomedical diagnostics, with the objective to replace animal experimentation.
A portable surface-enhanced Raman scattering sensor integrated with a lab-on-a-chip for field analysis
Ly Xuan Quang, Chaesung Lim, Gi Hun Seong, Jaebum Choo, Ki Jun Do and Seoung-Kyo Yoo,
Lab Chip, 2008, 8, 2214
DOI: 10.1039/b808835g
This paper represents a sensing system that uses a portable Raman spectrometer and a micropillar array chip. This offers a rapid and reproducible trace detection capability for hazardous materials in the field.
Back matter
Lab Chip, 2008, 8, 2220
DOI: 10.1039/b819448n
Back cover
Lab Chip, 2008, 8, 2223
DOI: 10.1039/b819449c
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