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Latest News
Chips & Tips: Integrated Reservoirs for PDMS Microfluidic Chips
22 April 2008
T. Liu and co-workers present integrated reservoirs for PDMS microfluidic chips.
On-chip suction stops worm wiggling
30 April 2008
Scientists in the US have developed a microfluidic method for immobilising worms in fractions of a second, allowing them to be used in high throughput studies of disease.
Finding the reaction rates for a particle-in-a-chip
23 April 2008
How does trapping a nanoparticle in a microdevice affect its reactions? US scientists are answering this question thanks to a straightforward method using fluorescent tags.
Chips & Tips: An easy temperature control system for syringe pumps
22 April 2008
Lorenzo Capretto and co-workers describe an easy temperature control system for syringe pumps.
Chips & Tips: Microfabrication Design Guidelines for Glass Micro- and Nano-fluidic Devices
18 April 2008
Dave Huber and Sumita Pennathur give some useful microfabrication design guidelines for glass micro- and nano-fluidic devices.
Nerve agent detector on a chip
16 April 2008
A microfluidic device that can identify exposure to sarin could help identify individuals needing treatment at sites of terrorist attack.
Cellular power plants fuel molecular motors
14 April 2008
Mitochondria have been used to power miniature motors for microfluidics.
3D chips assess drug viability
11 April 2008
Scientists in Germany have developed a new 3D chip for the rapid and cheap assessment of potential anticancer drugs.
Contents list for Lab on a Chip, issue 5, 2008
Front cover
Lab Chip, 2008, 8, 633
DOI: 10.1039/b805963m
Inside front cover
Lab Chip, 2008, 8, 634
DOI: 10.1039/b805964k
Contents and Chemical Technology
Lab Chip, 2008, 8, 635
DOI: 10.1039/b805966g
Highlight
Research Highlights
Lab Chip, 2008, 8, 645
DOI: 10.1039/b805061a
Petra Dittrich reviews the current literature in miniaturisation and related technologies.
Focus
Improving fluorescence detection in lab on chip devices
Lab Chip, 2008, 8, 649
DOI: 10.1039/b805064n
Sumita Pennathur and Deborah Kuchnir Fygenson discuss possibilities for high-resolution and high-sensitivity fluorescence devices—part of a series of mini-reviews covering new trends in fundamental and applied research, and potential applications of miniaturised technologies.
Communications
Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation
Fei Zeng, Christopher B. Rohde and Mehmet Fatih Yanik,
Lab Chip, 2008, 8, 653
DOI: 10.1039/b804808h
We demonstrate a method for non-invasive and high-throughput on-chip immobilization of physiologically active C. elegans without the use of anesthesia or cooling, but with comparable stability even for the most demanding purposes.
Tuneable separation in elastomeric microfluidics devices
Jason P. Beech and Jonas O. Tegenfeldt,
Lab Chip, 2008, 8, 657
DOI: 10.1039/b719449h
We describe how the elastomeric properties of PDMS can be utilised to achieve tuneable particle separation in Deterministic Lateral Displacement devices via strain controlled alteration of inter-obstacle distances, a development that opens up new avenues toward more effective separation of particles in microfluidics devices.
A disposable planar peristaltic pump for lab-on-a-chip
Levent Yobas, Kum-Cheong Tang, Shien-Eit Yong and Eleana Kye-Zheng Ong,
Lab Chip, 2008, 8, 660
DOI: 10.1039/b720024b
The authors demonstrate a planar peristaltic pump suitable for microfluidic integration by employing readily-available stainless-steel ball bearings and permanent magnets as actuating elements on PDMS.
Papers
Fabrication of a modular tissue construct in a microfluidic chip
Derek A. Bruzewicz, Alison P. McGuigan and George M. Whitesides,
Lab Chip, 2008, 8, 663
DOI: 10.1039/b719806j
Packing 200 µm sized molded gel objects containing cells into microfluidic channels yields a porous assembly that can be continuously perfused with cell culture medium for three-dimensional cell culture on a chip.
All-terrain droplet actuation
Mohamed Abdelgawad, Sergio L. S. Freire, Hao Yang and Aaron R. Wheeler,
Lab Chip, 2008, 8, 672
DOI: 10.1039/b801516c
We report a new method for fluid manipulation on flexible surfaces. Droplets can move across a wide range of geometries, and can selectively enrich DNA from a heterogeneous mixture.
On a chip demonstration of a functional role for odorant binding protein in the preservation of olfactory receptor activity at high odorant concentration
Jasmina Vidic, Jeanne Grosclaude, Régine Monnerie, Marie-Annick Persuy, Karine Badonnel, Christine Baly, Monique Caillol, Loïc Briand, Roland Salesse and Edith Pajot-Augy,
Lab Chip, 2008, 8, 678
DOI: 10.1039/b717724k
An OBP specifically binds an OR. Odorant ligand binding to the receptor specifically releases the bound OBP. Moreover, OBP preserves OR activity at high odorant concentration, unravelling its functional role.
Lab-on-chip methodologies for the study of transport in porous media: energy applications
Viatcheslav Berejnov, Ned Djilali and David Sinton,
Lab Chip, 2008, 8, 689
DOI: 10.1039/b802373p
A lab-on-chip approach to the study of two-phase transport in porous media is demonstrated.
Microfluidic device for immunoassays based on surface plasmon resonance imaging
Yiqi Luo, Fang Yu and Richard N. Zare,
Lab Chip, 2008, 8, 694
DOI: 10.1039/b800606g
We describe a microfluidic chip having addressable gold spots, which are used to sense antigen–antibody recognition events by surface plasmon resonance imaging.
A membrane-based microfluidic device for controlling the flux of platelet agonists into flowing blood
Keith B. Neeves and Scott L. Diamond,
Lab Chip, 2008, 8, 701
DOI: 10.1039/b717824g
This paper describes a membrane-based method for controlling the flux of soluble agonists into flowing blood. The device allows for real-time measurements of cellular adhesion and aggregation, as well as post hoc microscopy for examining aggregate morphology.
Fast-lysis cell traps for chemical cytometry
Paul J. Marc, Christopher E. Sims, Mark Bachman, G. P. Li and Nancy L. Allbritton,
Lab Chip, 2008, 8, 710
DOI: 10.1039/b719301g
Electrically addressable cell traps were integrated with capillary electrophoresis for the analysis of the contents of single adherent cells.
Automated cell culture in high density tubeless microfluidic device arrays
Ivar Meyvantsson, Jay W. Warrick, Steven Hayes, Allyson Skoien and David J. Beebe,
Lab Chip, 2008, 8, 717
DOI: 10.1039/b715375a
Harnessing surface tension in microfluidic systems enables automation, improves ease-of-use and allows microfluidic manipulation of cells in multiple compartments in parallel.
A novel microfluidic driver via AC electrokinetics
Ching-Te Kuo and Cheng-Hsien Liu,
Lab Chip, 2008, 8, 725
DOI: 10.1039/b719968f
We present a novel method for manipulations of a microfluid and micro-object via the asymmetric capacitance/chemistry modulation for controlling local fluid.
Micropallet arrays with poly(ethylene glycol) walls
Yuli Wang, Georgina To'a Salazar, Jeng-Hao Pai, Hamed Shadpour, Christopher E. Sims and Nancy L. Allbritton,
Lab Chip, 2008, 8, 734
DOI: 10.1039/b800286j
Arrays of poly(ethylene glycol) microwells with a removable base were used to isolate and clone cells following release of the lower microwell surface with attached cells.
An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models
Hiroshi Kimura, Takatoki Yamamoto, Hitomi Sakai, Yasuyuki Sakai and Teruo Fujii,
Lab Chip, 2008, 8, 741
DOI: 10.1039/b717091b
We present an integrated multi-functional microfluidic device having two-compartments separated by a semipermeable membrane with on-chip pumping and detection functions for long-term perfusion cell culture and monitoring of polarized transportation activity of intestinal tissue models.
Microcirculation within grooved substrates regulates cell positioning and cell docking inside microfluidic channels
Amir Manbachi, Shamit Shrivastava, Margherita Cioffi, Bong Geun Chung, Matteo Moretti, Utkan Demirci, Marjo Yliperttula and Ali Khademhosseini,
Lab Chip, 2008, 8, 747
DOI: 10.1039/b718212k
The integrated computational and experimental study suggests that the microcirculation within microfluidic grooved channels influences cell positioning and cell docking.
Numerical design of electrical-mechanical traps
Duc Vinh Le, Carlos Rosales, Boo Cheong Khoo and Jaime Peraire,
Lab Chip, 2008, 8, 755
DOI: 10.1039/b718153a
Effects of electrical and hydrodynamic forces on deformable cells are investigated in hybrid electrical-mechanical traps using the coupled IIM-BEM numerical technique. The technique makes useful quantitative predictions of the performance of different trap designs.
Ultrafast active mixer using polyelectrolytic ion extractor
Honggu Chun, Hee Chan Kim and Taek Dong Chung,
Lab Chip, 2008, 8, 764
DOI: 10.1039/b715229a
We present a low voltage, straight/smooth surface, and efficient active micromixer which allows selective RBC lysis. The mixing principle is based on alternative ion depletion-enrichment using a pair of positively charged polyelectrolytic gel electrodes (pPGEs).
Millisecond treatment of cells using microfluidic devices via two-step carrier-medium exchange
Masumi Yamada, Jun Kobayashi, Masayuki Yamato, Minoru Seki and Teruo Okano,
Lab Chip, 2008, 8, 772
DOI: 10.1039/b718281c
By rapidly exchanging the carrier-medium of cells twice, cells are exposed to a flow containing a chemical for cell treatment for a limited span of time.
Fourier microfluidics
Y. Xie, Y. Wang, L. Chen and C. H. Mastrangelo,
Lab Chip, 2008, 8, 779
DOI: 10.1039/b718376c
We present a new experimental technique for the separation of dynamic chemical signals with differing frequency domain charateristics.
Immobilization of DNAzyme catalytic beacons on PMMA for Pb2+ detection
Tulika S. Dalavoy, Daryl P. Wernette, Maojun Gong, Jonathan V. Sweedler, Yi Lu, Bruce R. Flachsbart, Mark A. Shannon, Paul W. Bohn and Donald M. Cropek,
Lab Chip, 2008, 8, 786
DOI: 10.1039/b718624j
We exploit biotin–streptavidin affinity to immobilize fluorescently labeled lead specific DNAzyme catalytic beacons within PMMA microfluidic channels to create a regenerable platform for real time determination of lead in water.
High-sensitivity, disposable lab-on-a-chip with thin-film organic electronics for fluorescence detection
Andrea Pais, Ansuman Banerjee, David Klotzkin and Ian Papautsky,
Lab Chip, 2008, 8, 794
DOI: 10.1039/b715143h
We report a method for high sensitivity on-chip fluorescence detection using a thin-film organic light-emitting diode (OLED) excitation source and an organic photodiode (OPD) detector.
Electrokinetic sorting and collection of fractions for preparative capillary electrophoresis on a chip
Dawid R. Zalewski, Stefan Schlautmann, Richard B. M. Schasfoort and Han J. G. E. Gardeniers,
Lab Chip, 2008, 8, 801
DOI: 10.1039/b717785b
We present a method for fast, accumulative fraction collection for capillary electrophoresis and discuss optimization methodology for such a system.
Technical Notes
Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber
Kwang Ho Cheong, Dong Kee Yi, Jeong-Gun Lee, Jong-Myeon Park, Min Jun Kim, Joshua B. Edel and Christopher Ko,
Lab Chip, 2008, 8, 810
DOI: 10.1039/b717382b
The unique optothermal property of Au nanorods was successively adopted to achieve a one step real-time PCR system for pathogen detection in a microchip without removing or changing reagents.
Gas–liquid selective oxidations with oxygen under explosive conditions in a micro-structured reactor
Arnaud Leclerc, Mohamad Alamé, Daniel Schweich, Patrick Pouteau, Cyril Delattre and Claude de Bellefon,
Lab Chip, 2008, 8, 814
DOI: 10.1039/b717985e
The gas–liquid oxidation of cyclohexane is performed at high temperature (>200 °C) and pressure (up to 25 bar) using pure oxygen in a Pyrex capped silicon etched microreactor which allows convenient screen reaction conditions well above the flammability limit.
Pinched flow fractionation devices for detection of single nucleotide polymorphisms
Asger Vig Larsen, Lena Poulsen, Henrik Birgens, Martin Dufva and Anders Kristensen,
Lab Chip, 2008, 8, 818
DOI: 10.1039/b802268b
We demonstrate a new and flexible microfluidic based method for genotyping single nucleotide polymorphisms (SNPs).
Coupling confocal fluorescence detection and recirculating microfluidic control for single particle analysis in discrete nanoliter volumes
C. M. Puleo, H. C. Yeh, K. J. Liu and T. H. Wang,
Lab Chip, 2008, 8, 822
DOI: 10.1039/b717941c
In this technical note we perform a single particle QD-FRET DNA assay within 5 nL volumes by increasing measurement efficiency through microfluidic recirculation.
Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk
Hong Chen, Lin Wang and Paul C. H. Li,
Lab Chip, 2008, 8, 826
DOI: 10.1039/b719846a
A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report.
Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection
Christophe Lay, Cheng Yong Teo, Liang Zhu, Xue Li Peh, Hong Miao Ji, Bi-Rong Chew, Ramana Murthy, Han Hua Feng and Wen-Tso Liu,
Lab Chip, 2008, 8, 830
DOI: 10.1039/b800015h
Enhanced HTML article available
A multi-layered ultra-fine microfiltration device with bypasses combined with a fluorescence based cell labeling method was developed to trap and detect bacterial cells without occurrences of clogging.
Back matter
Lab Chip, 2008, 8, 834
DOI: 10.1039/b805967p
Back cover
Lab Chip, 2008, 8, 835
DOI: 10.1039/b805968n
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