Issue 9, 2007
From the journal:

Lab on a Chip

Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites†

Ljiljana Puskar,a   Rudolf Tuckermann,b   Torsten Frosch,c   Jürgen Popp,c   Vanalysa Ly,a   Don McNaughtona  and  Bayden R. Wood*a  

* Corresponding authors

a Centre for Biospectroscopy and School of Chemistry, Monash University, Wellington Road, Victoria, Australia
E-mail: bayden.wood@sci.monash.edu.au
Fax: +613 9905 4597
Tel: +613 9905 4544

b Universität Göttingen, Institut für Physiische Chemie, Tammannstr. 6, Göttingen, Germany
E-mail: rtucker@gwdg.de
Fax: +49 551 39 3117
Tel: +49 551 39 3126

c Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, Jena, Germany
E-mail: juergen.popp@uni-jena.de
Fax: +49 3641 94 8302
Tel: +49 3641 94 8320

Abstract

Methods to probe the molecular structure of living cells are of paramount importance in understanding drug interactions and environmental influences in these complex dynamical systems. The coupling of an acoustic levitation device with a micro-Raman spectrometer provides a direct molecular probe of cellular chemistry in a containerless environment minimizing signal attenuation and eliminating the affects of adhesion to walls and interfaces. We show that the Raman acoustic levitation spectroscopic (RALS) approach can be used to monitor the heme dynamics of a levitated 5 µL suspension of red blood cells and to detect hemozoin in malaria infected cells. The spectra obtained have an excellent signal-to-noise ratio and demonstrate for the first time the utility of the technique as a diagnostic and monitoring tool for minute sample volumes of living animal cells.

Graphical abstract: Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites

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Article information

DOI
https://doi.org/10.1039/B706997A
Article type
Paper
Submitted
09 May 2007
Accepted
10 Jul 2007
First published
03 Aug 2007

Lab Chip, 2007,7, 1125-1131

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Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites

L. Puskar, R. Tuckermann, T. Frosch, J. Popp, V. Ly, D. McNaughton and B. R. Wood, Lab Chip, 2007, 7, 1125 DOI: 10.1039/B706997A

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