Issue 3, 2008
From the journal:

Lab on a Chip

Microfluidic depletion of endothelial cells, smooth muscle cells, and fibroblasts from heterogeneous suspensions†

Brian D. Plouffe,a   Milica Radisicbc  and  Shashi K. Murthy*a  

* Corresponding authors

a Department of Chemical Engineering, Northeastern University, Boston, Massachusetts, USA
E-mail: smurthy@coe.neu.edu
Fax: +1 (617) 373-2209
Tel: +1 (617) 373-4017

b Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

c Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada

Abstract

Interactions between ligands and cell surface receptors can be exploited to design adhesion-based microfluidic cell separation systems. When ligands are immobilized on the microfluidic channel surfaces, the resulting cell capture devices offer the typical advantages of small sample volumes and low cost associated with microfluidic systems, with the added benefit of not requiring complex fabrication schemes or extensive operational infrastructure. Cell–ligand interactions can range from highly specific to highly non-specific. This paper describes the design of an adhesion-based microfluidic separation system that takes advantage of both types of interactions. A 3-stage system of microfluidic devices coated with the tetrapeptides arg-glu-asp-val (REDV), val-ala-pro-gly (VAPG), and arg-gly-asp-ser (RGDS) is utilized to deplete a heterogeneous suspension containing endothelial cells, smooth muscle cells, and fibroblasts. The ligand-coated channels together with a large surface area allow effective depletion of all three cell types in a stagewise manner.

Graphical abstract: Microfluidic depletion of endothelial cells, smooth muscle cells, and fibroblasts from heterogeneous suspensions

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Article information

DOI
https://doi.org/10.1039/B715707J
Article type
Paper
Submitted
11 Oct 2007
Accepted
10 Jan 2008
First published
14 Feb 2008

Lab Chip, 2008,8, 462-472

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Microfluidic depletion of endothelial cells, smooth muscle cells, and fibroblasts from heterogeneous suspensions

B. D. Plouffe, M. Radisic and S. K. Murthy, Lab Chip, 2008, 8, 462 DOI: 10.1039/B715707J

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