RSC Publishing


Publishing

 

Cover image for Lab on a Chip, select for current issue

Lab on a Chip

Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering



Subscribers

Non-subscribers

Free access



Paper

Lab Chip, 2008, 8, 1048 - 1055, DOI: 10.1039/b802037j

Microfluidic add-on for standard electrophysiology chambers

Javeed Shaikh Mohammed, Hector Hugo Caicedo, Christopher P. Fall and David T. Eddington

We have developed a microfluidic brain slice device (BSD) that marries an off-the shelf brain slice perfusion chamber with an array of microfluidic channels set into the bottom surface of the chamber substrate. As this device is created through rapid prototyping, once optimized, it is trivial to replicate and share the devices with other investigators. The device integrates seamlessly into standard physiology and imaging chambers and it is immediately available to the whole slice physiology community. With this technology we can address the flow of neurochemicals and any other soluble factors to precise locations in the brain slice with the temporal profile we choose. Dopamine (DA) was chosen as a model neurotransmitter and we have quantified delivery in brain tissue using cyclic voltammetry (CV) and fluorescence imaging.

Graphical abstract image for this article (ID: b802037j)