Communication

Lab Chip, 2008, 8, 1617 - 1620, DOI: 10.1039/b807374k

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Formation of artificial lipid bilayers using droplet dielectrophoresis

Sara Aghdaei, Mairi E. Sandison, Michele Zagnoni, Nicolas G. Green and Hywel Morgan

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We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

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