Issue 8, 2009
From the journal:

Lab on a Chip

Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood†

Keiichi Hatakeyama,a   Tsuyoshi Tanaka,a   Masahiro Sawaguchi,b   Akihito Iwadate,b   Yasushi Mizutani,b   Kazuhiro Sasaki,b   Naofumi Tateishib  and  Tadashi Matsunaga*a  

* Corresponding authors

a Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, Japan
E-mail: tmatsuna@cc.tuat.ac.jp
Fax: +81 42-385-7713
Tel: +81 42-388-7020

b Casio Computer Co. Ltd., 6-2, Honmachi 1-chome, Shibuya-ku, Tokyo, Japan

Abstract

This work describes a novel microfluidic device using a thin film transistor (TFT) photosensor integrating a microfluidic channel, a DNA chip platform, and a photodetector for the discrimination of single nucleotide polymorphisms (SNPs). A DNA-arrayed TFT photosensor was used as a DNA chip platform and photo detecting device. Chemiluminescence was used for DNA sensing because chemiluminescence provides higher sensitivity and requires simpler instrumentation than fluorescence methods. The SNP of biotinylated target DNA was detected based on chemiluminescence by using horse radish peroxidase-conjugated streptavidin. The lower detection limit for a model biotinylated oligonucleotide (63-mer) was 0.5 nM, much lower than expected DNA concentrations in a practical application of this device. Furthermore, SNP detection in the aldehyde dehydrogenase 2 gene was successfully achieved using DNA-arrayed TFT photosensor without DNA extraction and DNA purification using PCR products. The assay was completed in less than one hour. Our technology will be a promising approach to developing a miniaturized, disposable DNA chip with high sensitivity.

Graphical abstract: Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood

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Article information

DOI
https://doi.org/10.1039/B817427J
Article type
Paper
Submitted
06 Oct 2008
Accepted
12 Dec 2008
First published
21 Jan 2009

Lab Chip, 2009,9, 1052-1058

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Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood

K. Hatakeyama, T. Tanaka, M. Sawaguchi, A. Iwadate, Y. Mizutani, K. Sasaki, N. Tateishi and T. Matsunaga, Lab Chip, 2009, 9, 1052 DOI: 10.1039/B817427J

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