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Microfluidic & nanofluidic technologies for chemistry, physics, biology, and bioengineering
Paper
Lab Chip, 2009, 9, 1676 - 1680, DOI: 10.1039/b901999e
Microfluidic chip for combinatorial mixing and screening of assays
Benjamin R. Schudel, Charles J. Choi, Brian T. Cunningham and Paul J. A. Kenis
This paper reports the design, fabrication and validation of a microfluidic well plate for combinatorial screening applications. Each well within the array is comprised of two 200 picoliter compartments that each contain a photonic crystal biosensor to enable the on-chip, in situ detection of (bio-) molecular binding events. This microfluidic chip utilizes arrays of Actuate-to-Open valves to isolate all compartments, which allows the chip to be decoupled from pneumatic control lines and thus to be transported freely between filling, sensing and characterization platforms. A proof-of-principle 4 × 4 protein/antibody binding assay was performed to demonstrate the discrete mixing and on-chip sensing capabilities.
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