Issue 20, 2010
From the journal:

Lab on a Chip

Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNAmelting curves†

Pavel Neuzil,‡*a   Fang Cheng,§a   Jeffrey Bo Woon Soon,a   Lan Liu Qiana  and  Julien Rebouda  

* Corresponding authors

a Institute of Microelectronics, 11 Science Park Road, Singapore
E-mail: pavel@kist-europe.de

Abstract

This report introduces a bleaching-independent temperature measurement method based on the analysis of the fluorescence emitted during the melting of DNA molecules with the SYBR-Green I intercalator, in a microvolume where the strong non-linearity of the signal is used to eliminate the photobleaching effect as well as to determine the heat transfer rate between a heater and the sample and the temperature non-uniformity within the sample.

Graphical abstract: Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves

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Article information

DOI
https://doi.org/10.1039/C005243D
Article type
Technical Note
Submitted
30 Apr 2010
Accepted
02 Aug 2010
First published
07 Sep 2010

Lab Chip, 2010,10, 2818-2821

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Non-contact fluorescent bleaching-independent method for temperature measurement in microfluidic systems based on DNA melting curves

P. Neuzil, F. Cheng, J. B. W. Soon, L. L. Qian and J. Reboud, Lab Chip, 2010, 10, 2818 DOI: 10.1039/C005243D

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