Issue 23, 2010
From the journal:

Lab on a Chip

Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

Aaron T. Ohta,†*a   Maurice Garcia,†b   Justin K. Valley,c   Lia Banie,b   Hsan-Yin Hsu,c   Arash Jamshidi,c   Steven L. Neale,c   Tom Lueb  and  Ming C. Wuc  

* Corresponding authors

a Department of Electrical Engineering, University of Hawaii at Manoa, Honolulu, HI, USA
E-mail: aohta@hawaii.edu
Fax: +1-808-956-3427
Tel: +1-808-956-8196

b Department of Urology, University of California, San Francisco, CA, USA

c Department of Electrical Engineering & Computer Sciences, University of California, Berkeley, CA, USA

Abstract

Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s−1, while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of −0.8 ± 1.0 µm s−1. Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies.

Graphical abstract: Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

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Article information

DOI
https://doi.org/10.1039/C0LC00072H
Article type
Paper
Submitted
04 Jun 2010
Accepted
12 Aug 2010
First published
08 Sep 2010

Lab Chip, 2010,10, 3213-3217

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Motile and non-motile sperm diagnostic manipulation using optoelectronic tweezers

A. T. Ohta, M. Garcia, J. K. Valley, L. Banie, H. Hsu, A. Jamshidi, S. L. Neale, T. Lue and M. C. Wu, Lab Chip, 2010, 10, 3213 DOI: 10.1039/C0LC00072H

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