Issue 10, 2009
From the journal:

Molecular BioSystems

Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring†‡

Josselin Noirel,a   Saw Yen Ow,a   Guido Sanguinettiab  and  Phillip C. Wright*a  

* Corresponding authors

a ChELSI Research Institute, The Sheffield Bioincubator, Department of Chemical and Process Engineering, University of Sheffield, Mappin St, Sheffield, United Kingdom
E-mail: p.c.wright@sheffield.ac.uk

b Department of Computer Science, University of Sheffield, 211 Portobello St, Sheffield, United Kingdom

Abstract

We present a systems biology approach to study the global metabolic effects of the insertion of synthetic circuits in a cellular chassis. Our approach combines high-throughput proteomics with the MMG probabilistic tool, which integrates the data with the metabolic circuit’s topology. We present a theoretical analysis of the foundations of our approach, as well as experimental results on a mutant strain of Escherichia coli where a light-receptor circuit was inserted and coupled with lactose metabolism. Our results show that the systems approach manages to extract meaningful information from the proteomic data that cannot be recovered by naive thresholding of the data. This tool can be used to characterise the relationship between new circuits and chassis in synthetic biology applications.

Graphical abstract: Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring

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Article information

DOI
https://doi.org/10.1039/B904729H
Article type
Paper
Submitted
09 Mar 2009
Accepted
28 May 2009
First published
07 Jul 2009

Mol. BioSyst., 2009,5, 1214-1223

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Systems biology meets synthetic biology: a case study of the metabolic effects of synthetic rewiring

J. Noirel, S. Y. Ow, G. Sanguinetti and P. C. Wright, Mol. BioSyst., 2009, 5, 1214 DOI: 10.1039/B904729H

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