RSC Publishing


Publishing

 

Cover image for Molecular BioSystems, select for current issue

Molecular BioSystems

Research at the interface between chemistry and the -omic sciences and systems biology.



Subscribers

  • PDF
  • HTML article

Non-subscribers

Free access



Review

Mol. BioSyst., 2009, 5, 1112 - 1121, DOI: 10.1039/b909534a

Receptor tyrosine kinase signaling: a view from quantitative proteomics

Joern Dengjel, Irina Kratchmarova and Blagoy Blagoev

Growth factor receptor signaling via receptor tyrosine kinases (RTKs) is one of the basic cellular communication principals found in all metazoans. Extracellular signals are transferred via membrane spanning receptors into the cytoplasm, reversible tyrosine phosphorylation being the hallmark of all RTKs. In recent years proteomic approaches have yielded detailed descriptions of cellular signaling events. Quantitative proteomics is able to characterize the exact position and strength of post-translational modifications (PTMs) providing essential information for understanding the molecular basis of signal transduction. Numerous new post-translational modification sites have been identified by quantitative mass spectrometry-based proteomics. In addition, plentiful new players in signal transduction have been identified underlining the complexity and the modular architecture of most signaling networks. In this review, we outline the principles of signal transduction via RTKs and highlight some of the new insights obtained from proteomic approaches such as protein microarrays and quantitative mass spectrometry.

Graphical abstract image for this article (ID: b909534a)