Parallel on-line detection of a methylbismuth species by hyphenated GC/EI-MS/ICP-MS technique as evidence for bismuth methylation by human hepatic cells

Markus Hollmann; Jens Boertz; Elke Dopp; Joerg Hippler; Alfred Vitalis Hirner

doi:10.1039/B911945K

Parallel on-line detection of a methylbismuth species by hyphenated GC/EI-MS/ICP-MS technique as evidence for bismuth methylation by human hepatic cells†

Markus Hollmann,*^a Jens Boertz,‡^a Elke Dopp,^b Joerg Hippler^a and Alfred Vitalis Hirner^a

* Corresponding authors

^a Institute of Environmental Analytical Chemistry, University of Duisburg-Essen, Universitaetsstrasse 3-5, 45141 Essen, Germany
E-mail: markus_hollmann@uni-due.de
Fax: +49 (0)201 1833951
Tel: +49 (0)201 1833238

^b Institute of Hygiene and Occupational Medicine, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany
E-mail: elke.dopp@uni-due.de
Fax: +49 (0)201 7234546
Tel: + 49( 0)201 7234578

Abstract

Methylation of metal(loid)s by bacteria or even mammals is a well known process that can lead to increased toxicity for humans. Nevertheless, reliable analytical techniques and tools are indispensable in speciation analysis of trace elements, especially since environmental or biological samples are usually characterised by complex matrices. Here the methylating capability of hepatic cells was observed in vitro. HepG2 cells were incubated with colloidal bismuth subcitrate, bismuth cysteine and bismuth glutathione, respectively for a period of 24 h. For identification the cell lysate was ethylated by sodium tetraethyl borate under neutral conditions. After cryo focussing by purge and trap, the bismuth speciation was carried out viaGC/EI-MS/ICP-MS. Colloidal bismuth subcitrate and bismuth cysteine were methylated by HepG2 cells, while no methylated bismuth species was detected after incubation with bismuth glutathione.

Metallomics

Parallel on-line detection of a methylbismuth species by hyphenated GC/EI-MS/ICP-MS technique as evidence for bismuth methylation by human hepatic cells†

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