A proteome investigation of roxarsone degradation by Alkaliphilus oremlandii strain OhILAs

Peter Chovanec; John F. Stolz; Partha Basu

doi:10.1039/B915479E

A proteome investigation of roxarsone degradation by Alkaliphilus oremlandii strain OhILAs

Peter Chovanec,^ab John F. Stolz*^b and Partha Basu*^a

* Corresponding authors

^a Department of Chemistry and Biochemistry, Duquesne University, 600 Forbes Ave, Pittsburgh PA 15282, USA
E-mail: basu@duq.edu

^b Department of Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh PA 15282, USA

Abstract

Clostridial species predominate in both chicken gastrointestinal tract as well as litter where the organoarsenical roxarsone (3-nitro 4-hydroxybenzenearsonic acid) is anaerobically transformed releasing the more recognized toxic inorganic arsenic. 2D-gel electrophoresis and mass spectrometry were used to evaluate the changes in protein expression of Alkaliphilus oremlandii in response to different growth conditions (e.g., terminal electron acceptors) in order to explore the mechanism of microbial biotransformation of roxarsone. Aldehyde ferredoxin oxidoreductase, the enzyme that belongs to the xanthine oxidase family of molybdoenzymes was significantly overexpressed in the presence of roxarsone suggesting a role in the anaerobicmetabolism of this substituted nitrophenol.

This article is part of the themed collection: 2009 International Symposium on Metallomics, Cincinnati, USA

Metallomics

A proteome investigation of roxarsone degradation by Alkaliphilus oremlandii strain OhILAs

Abstract

Article information

Download Citation

Search articles by author

Spotlight

Advertisements