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Paper
Org. Biomol. Chem., 2006, 4, 1887 - 1895, DOI: 10.1039/b601540a
A paramagnetic contrast agent with myeloperoxidase-sensing properties
Manel Querol, John W. Chen and Alexei A. Bogdanov Jr.
Myeloperoxidase (MPO) is an essential component of inflammatory response in norm and pathology. With an ultimate goal of non-invasive imaging of MPO we used a gadolinium-chelating bis(5-hydroxytrytamide) derivative of diethylenetetraamine pentaacetic acid (L1–Gd3+salt) as a paramagnetic sensor of enzymatic activity. We tested whether L1–Gd3+ is active in reducing the oxidized form of myeloperoxidase, generated as a result of hydrogen peroxide reduction by the enzyme. We expected that upon activation by MPO/H2O2 L1–Gd3+ would not only oligomerise but also bind to other macromolecules present in the media and that the overall process will give rise to a net T1-weighted MRI signal increase.
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