Issue 5, 2010
From the journal:

Soft Matter

Neutron reflectometry of supported hybrid bilayers with inserted peptide

Matthew B. Smith,a   Duncan J. McGillivray,d   Jan Genzer,a   Mathias Löschebc  and  Peter K. Kilpatrick*ae  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina, USA
E-mail: peter.kilpatrick@nd.edu

b National Institute of Standards and Technology (NIST) Center for Neutron Research (NCNR), Gaithersburg, Maryland, USA

c Physics Department, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA

d Department of Chemistry, The University of Auckland, Auckland, New Zealand

e Department of Chemical and Biomolecular Engineering, University of Notre Dame, 257 Fitzpatrick Hall, Notre Dame, IN, USA

Abstract

The insertion of a synthetic amphiphilic, α-helical peptide into a supported hybrid bilayer membrane (HBM) was studied by neutron reflectometry to elucidate the resulting nanostructure. The HBM consisted of a self-assembled monolayer of perdeuterated octadecanethiol on gold and an overlying leaflet of acyl-deuterated phosphatidylcholine (d-DMPC). Using contrast variation, several reflectivity spectra were recorded for each step of film fabrication, and simultaneously modeled. This analysis indicated that peptide insertion into the DMPC lipid leaflet is the likeliest mode of incorporation.

Graphical abstract: Neutron reflectometry of supported hybrid bilayers with inserted peptide

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Article information

DOI
https://doi.org/10.1039/B915800F
Article type
Communication
Submitted
03 Aug 2009
Accepted
07 Dec 2009
First published
04 Jan 2010

Soft Matter, 2010,6, 862-865

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Neutron reflectometry of supported hybrid bilayers with inserted peptide

M. B. Smith, D. J. McGillivray, J. Genzer, M. Lösche and P. K. Kilpatrick, Soft Matter, 2010, 6, 862 DOI: 10.1039/B915800F

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