Issue 15, 2010
From the journal:

Soft Matter

Directed assembly of yeast cells into living yeastosomes by microbubble templating†

Marie-Laure Brandy,a   Olivier J. Cayre,b   Rawil F. Fakhrullin,ac   Orlin D. Velevd  and  Vesselin N. Paunov*a  

* Corresponding authors

a Surfactant and Colloid Group, Department of Chemistry, The University of Hull, Hull, UK
E-mail: V.N.Paunov@chem.hull.ac.uk
Fax: +44 (0)1482 466410
Tel: +44 (0)1482 465220

b School of Process, Environmental and Materials Engineering, University of Leeds, Leeds, UK

c Department of Biochemistry, Kazan (Idel-Ural) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan

d Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, USA

Abstract

We report the fabrication of yeastosomes—novel multicellular assemblies, which consist of a spherical monolayer of living yeast cells held together by colloid interactions. Our method is based on templating of microbubbles with cells coated with cationic polyelectrolyte and the layer-by-layer technique. We show the mechanism of formation of yeastosomes which involves a gradual dissolution of the air-cores and their infusion with water. We demonstrate that the cells remain viable in the yeastosome structures. Yeastosomes and similar structures may find applications in the development of novel symbiotic bio-structures, artificial multicellular organisms and in tissue engineering. The unusual structure of these multicellular assemblies resembles the primitive forms of multicellular species, like Volvox, and other algae and could be regarded as one possible stage of the evolutionary development of multicellularity.

Graphical abstract: Directed assembly of yeast cells into living yeastosomes by microbubble templating

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Article information

DOI
https://doi.org/10.1039/C0SM00003E
Article type
Paper
Submitted
18 Feb 2010
Accepted
01 Apr 2010
First published
17 May 2010

Soft Matter, 2010,6, 3494-3498

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Directed assembly of yeast cells into living yeastosomes by microbubble templating

M. Brandy, O. J. Cayre, R. F. Fakhrullin, O. D. Velev and V. N. Paunov, Soft Matter, 2010, 6, 3494 DOI: 10.1039/C0SM00003E

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