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Miniaturised sewing machines
23 June 2008
Single threads of DNA can now be manipulated using miniaturised hooks and bobbins, thanks to Japanese scientists
Sizing up proteins on-chip
13 June 2008
US scientists have formed gels on-chip for easier and cheaper protein separation
New journal announced: Integrative Biology
12 June 2008
January 2009 will see Integrative Biology: Quantitative biosciences from nano to macro, launched by RSC Publishing
Contents list for Lab on a Chip, issue 7, 2008
Front cover
Lab Chip, 2008, 8, 993
DOI: 10.1039/b809854a
Inside front cover
Lab Chip, 2008, 8, 994
DOI: 10.1039/b809856p
Contents and Chemical Technology
Lab Chip, 2008, 8, 995
DOI: 10.1039/b809857n
Highlight
Research Highlights
Lab Chip, 2008, 8, 1007
DOI: 10.1039/b808698m
Petra Dittrich reviews the current literature in miniaturisation and related technologies.
Focus
Multiphase flow in lab on chip devices: A real tool for the future?
Lab Chip, 2008, 8, 1010
DOI: 10.1039/b808974b
Sumita Pennathur, Lingling Shui, Jan Eijkel and Albert van den Berg discuss the future direction of microfluidic multiphase flow—part of a series of mini-reviews covering new trends in fundamental and applied research, and potential applications of miniaturised technologies.
Tutorial Review
Cell research with physically modified microfluidic channels: A review
Sun Min Kim, Sung Hoon Lee and Kahp Yang Suh,
Lab Chip, 2008, 8, 1015
DOI: 10.1039/b800835c
An overview of the use of physically modified microfluidic channels towards cell research is presented here. Fabrication methods as well as mechanical operation principles aided by structural modification of the channels are demonstrated with exemplary works.
Communications
Monolithic photolithographically patterned Fluorocur
PFPE membrane valves and pumps for in situ planetary exploration
Peter A. Willis, Frank Greer, Michael C. Lee, J. Anthony Smith, Victor E. White, Frank J. Grunthaner, Jacob J. Sprague and Jason P. Rolland,
Lab Chip, 2008, 8, 1024
DOI: 10.1039/b804265a
Photolithographically defined monolithic membrane valves utilizing perfluoropolyether were fabricated and characterized to be essentially unaltered after one million actuations and exposure to the environmental stresses associated with planetary exploration.
A refillable microfabricated drug delivery device for treatment of ocular diseases
Ronalee Lo, Po-Ying Li, Saloomeh Saati, Rajat Agrawal, Mark S. Humayun and Ellis Meng,
Lab Chip, 2008, 8, 1027
DOI: 10.1039/b804690e
An implantable and refillable manually-actuated microfabricated drug delivery device is presented as a new approach for targeted delivery of therapeutic compounds to ocular tissue.
Continuous molecular enrichment in microfluidic systems
Oliver K. Castell, Christopher J. Allender and David A. Barrow,
Lab Chip, 2008, 8, 1031
DOI: 10.1039/b800521d
We have demonstrated for the first time highly efficient molecular extractions in continuous flow microfluidic systems utilising the rapid mixing properties of biphasic segmented flow in conjunction with suspended micro-particulate adsorbents to enhance extraction.
Papers
Living cantilever arrays
for characterization of mass of single live cells in fluids
Kidong Park, Jaesung Jang, Daniel Irimia, Jennifer Sturgis, James Lee, J. Paul Robinson, Mehmet Toner and Rashid Bashir,
Lab Chip, 2008, 8, 1034
DOI: 10.1039/b803601b
A single HeLa cell was captured and cultured on a silicon cantilever and then its mass was measured by monitoring the change in resonant frequency of the cantilever.
Microfabrication of an asymmetric, multi-layered microdevice for controlled release of orally delivered therapeutics
Kristy M. Ainslie, Casey M. Kraning and Tejal A. Desai,
Lab Chip, 2008, 8, 1042
DOI: 10.1039/b800604k
A photolithographically patterned, multi-layered poly(ethylene glycol) dimethacrylate hydrogel laden with proteins or chemotherapeutics was introduced into a micro-patterned multi-layer SU-8 microdevice.
Microfluidic add-on for standard electrophysiology chambers
Javeed Shaikh Mohammed, Hector Hugo Caicedo, Christopher P. Fall and David T. Eddington,
Lab Chip, 2008, 8, 1048
DOI: 10.1039/b802037j
We have developed a microfluidic brain slice device (
BSD) that marries an off-the shelf brain slice perfusion chamber with an array of microfluidic channels set into the bottom surface of the chamber substrate to address the flow of neurochemicals and any other soluble factors to precise locations in the brain slice.
Stop-flow lithography to generate cell-laden microgel particles
Priyadarshi Panda, Shamsher Ali, Edward Lo, Bong Geun Chung, T. Alan Hatton, Ali Khademhosseini and Patrick S. Doyle,
Lab Chip, 2008, 8, 1056
DOI: 10.1039/b804234a
Stop-flow lithography (SFL) is used to generate cell-laden microgel particles which can be used for various biomedical applications such as tissue engineered constructs and multi-cell drug assays.
Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry
Michael J. Rosenbluth, Wilbur A. Lam and Daniel A. Fletcher,
Lab Chip, 2008, 8, 1062
DOI: 10.1039/b802931h
Measurement of blood cell mechanics is enabled by flowing cells through a parallel network of microchannels. Here, an acute myeloid leukemia cell from a patient who died of leukostasis, a complication of acute leukemia, occludes a microchannel, mimicking in vivo microvascular occlusion.
Electrically controlled microvalves to integrate microchip polymerase chain reaction and capillary electrophoresis
Govind V. Kaigala, Viet N. Hoang and Christopher J. Backhouse,
Lab Chip, 2008, 8, 1071
DOI: 10.1039/b802853b
A scalable, electrically-controlled microvalve is used to integrate genetic amplification and detection/analysis for medical diagnostic use.
Lateral-driven continuous dielectrophoretic microseparators for blood cells suspended in a highly conductive medium
Ki-Ho Han and A. Bruno Frazier,
Lab Chip, 2008, 8, 1079
DOI: 10.1039/b802321b
Red blood cells (RBCs) and white blood cells (WBCs) separation at the output of (A) the divergent type and (B) the convergent type lateral continuous DEP microseparators
Chemotaxis in microfluidic devices—a study of flow effects
Carsten Beta, Toni Fröhlich, Hendrik U. Bödeker and Eberhard Bodenschatz,
Lab Chip, 2008, 8, 1087
DOI: 10.1039/b801331d
Numerical finite element simulations reveal that the interplay of cell geometry and flow properties has a strong impact on the concentration profile across the surface of cells in microfluidic gradient mixers.
Two simple and rugged designs for creating microfluidic sheath flow
Peter B. Howell Jr, Joel P. Golden, Lisa R. Hilliard, Jeffrey S. Erickson, David R. Mott and Frances S. Ligler,
Lab Chip, 2008, 8, 1097
DOI: 10.1039/b719381e
Grooves in the top and bottom walls of microfluidic channels are used to create a very robust sheath flow system with as little as a single sheath flow inlet. Designs were demonstrated in PDMS using soft lithography and PMMA using micromilling and laser ablation. Such sheath flow systems are robust, resistant to clogging, and appropriate for microflow cytometry applications.
Gene transfer device utilizing micron-spiked electrodes produced by the self-organization phenomenon of Fe-alloy
Naoki Miyano, Yuuki Inoue, Yuji Teramura, Keisuke Fujii, Fujio Tsumori, Hiroo Iwata and Hidetoshi Kotera,
Lab Chip, 2008, 8, 1104
DOI: 10.1039/b718655j
A micro-spike is produced based on a self-organization phenomenon of a stainless steel. With the spike-projected device, we could transfer plasmid DNA into adherent HEK293 cells on the spiked-electrodes.
Drop-based microfluidic devices for encapsulation of single cells
Sarah Köster, Francesco E. Angilè, Honey Duan, Jeremy J. Agresti, Anton Wintner, Christian Schmitz, Amy C. Rowat, Christoph A. Merten, Dario Pisignano, Andrew D. Griffiths and David A. Weitz,
Lab Chip, 2008, 8, 1110
DOI: 10.1039/b802941e
Our approach utilizes a very robust and highly flexible modular device design which allows single-cell encapsulation, incubation, and handling. Individual hybridoma cells produce detectable amounts of antibodies within a drop in only a few hours and can subsequently be recovered and recultivated.
An integrated optofluidic platform for Raman-activated cell sorting
Adrian Y. Lau, Luke P. Lee and James W. Chan,
Lab Chip, 2008, 8, 1116
DOI: 10.1039/b803598a
This paper describes the development of an optofluidic Raman-activated cell sorting (RACS) device combining laser tweezers Raman spectroscopy (LTRS) with multichannel microfluidics for cell delivery, spectral analysis, and sorting of cells.
A practical guide to the staggered herringbone mixer
Manda S. Williams, Kenneth J. Longmuir and Paul Yager,
Lab Chip, 2008, 8, 1121
DOI: 10.1039/b802562b
An analytical model of mixing in the staggered herringbone mixer was developed, using theory and information from computational fluid dynamics (CFD) modeling, to describe performance and predict ideal operational parameters. This analytical model matches well with results from confocal microscopy and conventional CFD models.
PCR-based detection in a micro-fabricated platform
Shantanu Bhattacharya, Shuaib Salamat, Dallas Morisette, Padmapriya Banada, Demir Akin, Yi-Shao Liu, Arun K. Bhunia, Michael Ladisch and Rashid Bashir,
Lab Chip, 2008, 8, 1130
DOI: 10.1039/b802227e
A microfluidic device is presented for the capture, lysis, genetic amplification, and identification of Listeria monocytogenes V7 with high sensitivity and specificity in pure samples and in the presence of other bacteria.
Rapid and variable-volume sample loading in sieving electrophoresis microchips using negative pressure combined with electrokinetic force
Li-Ya Qi, Xue-Feng Yin, Lei Zhang and Min Wang,
Lab Chip, 2008, 8, 1137
DOI: 10.1039/b800085a
Rapid and variable-volume sample loading in sieving electrophoresis microchips by negative pressure combined with electrokinetic force using a simple and robust sampling device.
Optimization of sample transfer in two-dimensional microfluidic separation systems
Shuang Yang, Jikun Liu and Don L. DeVoe,
Lab Chip, 2008, 8, 1145
DOI: 10.1039/b801978a
Optimized interdimensional sample transfer in a 2-D microfluidic separation platform is achieved through the use of multidimensional backbiasing and an angled channel design.
The influence of membrane ion-permselectivity on electrokinetic concentration enrichment in membrane-based preconcentration units
Dzmitry Hlushkou, Rahul Dhopeshwarkar, Richard M. Crooks and Ulrich Tallarek,
Lab Chip, 2008, 8, 1153
DOI: 10.1039/b800549d
The presence of fixed charges on the backbone of a nanoporous membrane results in ion-permselectivity which adversely affects membrane performance in electrokinetic concentration enrichment due to concentration polarization induced by the applied electrical field in the adjoining bulk electrolyte solutions.
Effect of viscoelasticity on the flow pattern and the volumetric flow rate in electroosmotic flows through a microchannel
H. M. Park and W. M. Lee,
Lab Chip, 2008, 8, 1163
DOI: 10.1039/b800185e
Viscoelastic fluids such as biofluids may induce secondary flows in a straight microchannel, resulting in reduced solute dispersion as compared to the case of Newtonian fluids.
The change of activation energy in microchannel laminar flow as demonstrated by kinetic analysis of the DNA duplex–coil equilibrium
Kenichi Yamashita, Masaya Miyazaki, Yoshiko Yamaguchi, Hiroyuki Nakamura and Hideaki Maeda,
Lab Chip, 2008, 8, 1171
DOI: 10.1039/b800986d
The ability to change and regulate the activation energy of chemical reactions in microchannel laminar flow without the presence of catalysts is presented.
Acoustic resonances in straight micro channels: Beyond the 1D-approximation
S. M. Hagsäter, A. Lenshof, P. Skafte-Pedersen, J. P. Kutter, T. Laurell and H. Bruus,
Lab Chip, 2008, 8, 1178
DOI: 10.1039/b801028e
In this study we investigate an acoustic separator through micro-PIV analysis and numerical simulations.
Full-field photonic biosensors based on tunable bio-doped sol–gel glasses
Andreu Llobera, Victor J. Cadarso, Margarita Darder, Carlos Domínguez and César Fernández-Sánchez,
Lab Chip, 2008, 8, 1185
DOI: 10.1039/b801152d
Organic–inorganic sol–gel polymers doped with an enzyme were applied to the fabrication of a full-field generic photonic biosensor approach based on a strip waveguide configuration. Ultra-compact biosensor devices can be fabricated where both the transducer and the recognition elements are merged into one single microstructure.
Silane–dextran chemistry on lateral flow polymer chips for immunoassays
Christina Jönsson, Magnus Aronsson, Gerd Rundström, Christer Pettersson, Ib Mendel-Hartvig, Jimmy Bakker, Erik Martinsson, Bo Liedberg, Brian MacCraith, Ove Öhman and Jonas Melin,
Lab Chip, 2008, 8, 1191
DOI: 10.1039/b800297e
We demonstrate a new microfluidic platform in combination with a novel protocol for surface hydrophilization and antibody immobilization for immunoassays on cycloolefin-copolymer test chips.
Light-actuated high pressure-resisting microvalve for on-chip flow control based on thermo-responsive nanostructured polymer
Guofang Chen, Frantisek Svec and Daniel R. Knapp,
Lab Chip, 2008, 8, 1198
DOI: 10.1039/b803293a
Light actuation of a microfluidic valve is effected by heat induced opening of pores in a monolithic plug of crosslinked poly(N-isopropylacrylamide) attached to the wall.
Fourier transform to analyse reaction-diffusion dynamics in a microsystem
André Estévez-Torres, Thomas Le Saux, Charlie Gosse, Annie Lemarchand, Anne Bourdoncle and Ludovic Jullien,
Lab Chip, 2008, 8, 1205
DOI: 10.1039/b805412f
An integrated approach relying on a microsystem is introduced to easily extract, from a single experiment and with a global robust bi-exponential fit, an extensive set of thermodynamic, kinetic, and diffusion parameters governing associations in solution.
Potentiometric platform for the quantification of cellular potassium efflux
Silvia Generelli, Renaud Jacquemart, Nico F. de Rooij, Mario Jolicoeur, Milena Koudelka-Hep and Olivier T. Guenat,
Lab Chip, 2008, 8, 1210
DOI: 10.1039/b801042k
A microfluidic platform with ion-selective microelectrodes is used to monitor extracellular K+ effluxes in real time and follow cell swelling and necrosis induced by the modification of the extracellular osmolarity.
Technical Notes
Triggering vacuum capillaries for pneumatic pumping and metering liquids in point-of-care immunoassays
Kuo-Yao Weng, Nien-Jen Chou and Jya-Wei Cheng,
Lab Chip, 2008, 8, 1216
DOI: 10.1039/b801878b
Vacuum glass capillaries broken by external force such as finger pressure, generate the pneumatic forces to induce liquid flow in the fluidic system.
Concentration gradient generator using a convective–diffusive balance
Taekyu Kang, Jeahyeong Han and Ki Sung Lee,
Lab Chip, 2008, 8, 1220
DOI: 10.1039/b800859k
A novel way of establishing concentration gradients using a convective–diffusive balance in a counterflow configuration is described. Steady or temporal concentration gradient profiles can be predicted with a simple mathematical formula.
Back matter
Lab Chip, 2008, 8, 1223
DOI: 10.1039/b809858c
Back cover
Lab Chip, 2008, 8, 1227
DOI: 10.1039/b809859j
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