Raman bites into tooth decay

Mineral distribution signals help discriminate healthy enamel from cavities

Researchers in the US have adapted Raman imaging systems to demonstrate the technique’s potential for spotting warning signs of dental cavities.

Dental cavities come about when lactic acid, produced by oral bacterial, decomposes the protective mineral coating on teeth, leaving them vulnerable to decay. Detecting signs of rotting early is vital for successful treatment to prevent painful infections and loss of teeth, but they are often hard for a dentist to spot through visual inspection or x-ray images.

Raman spectroscopy is commonly used to assess the mineral content of bodily tissues as it is very sensitive to mineral crystals. However, classical Raman imaging uses linear laser light which produces an array of spectra and requires the sample to be moved with respect to the laser. This is time consuming and limits its clinically feasible.                                                              

Wide-field Raman imaging is being investigated by Ozan Akkus, and his team at Case Western Reserve University, as a more efficient method. Their most recent strategy combines wide-field Raman imaging with near infra-red lasers, special filters and 2D-charge-coupled device (CCD) cameras. It differs from classical Raman imaging as it only produces images of select wavenumbers of interest and defocusses the laser beam so larger areas can be analysed.

Areas of low and high mineral content are identified as weak and intense Raman signals, respectively, in a process that is 13 times faster than previously used Raman methods. Akkus stresses that ‘if dentists were able to image the mineral variation over the entire surface of a tooth, they would be able to identify and address any emerging problems’.

Jemma Kerns, a Raman spectroscopy expert at University College London, UK, comments that this application of Raman spectroscopic imaging, to identify mineralisation changes in dental lesions, is significant and will ‘allow for the development of better treatment options to directly benefit patients in the future.’

Akkus says that they now wish to increase the area that they can image and reduce the cost of the procedure in order to translate this concept into practice.


This paper is free to access until 22 May 2014. Download it here:

S Yang et al, Analyst, 2014, DOI: 10.1039/c4an00164h

Related Content

Patching up tooth enamel

25 January 2011 News Archive

news image

Scientists have been able to rebuild eroded tooth enamel with a hydrogel

Shell extract helps rebuild pearly whites

10 April 2013 Research

news image

Tooth enamel restoration inspired by nacre

Most Commented

New antibiotic picked from nose bacteria

27 July 2016 Research

news image

Discovery suggests human microbiome may be an untapped source of antimicrobial compounds

Perovskite boosts silicon solar cell efficiency

25 November 2015 News and Analysis

news image

Silicon industry will be ‘beating a path to the door’ of inventors, says scientist