Antibacterial nanoparticles from bacteria
04 June 2010
Scientists have found that silver nanoparticles made using bacteria have better antibacterial properties than their chemically synthesised counterparts.
Mitchel Doktycz and colleagues at Oak Ridge National Laboratory and the University of Tennessee in the US incubated Shewanella oneidensis bacteria with silver nitrate solution to produce uniform sized silver nanoparticles.
The team's biogenic-Ag nanoparticles were of regular size and well-defined composition
© Environ. Sci. Technol.
The team compared the toxicity of their biogenic nanoparticles to two types of chemically synthesised nanoparticles - bare silver nanoparticles (colloidal-Ag) and fatty acid coated silver nanoparticles (oleate-Ag). Assays carried out on Escherichia coli, Bacillus subtilis and S. oneidensis bacteria showed that for all three strains the biogenic nanoparticles had the highest toxicity levels. Colloidal-Ag was less potent, while oleate-Ag was not toxic to any of the bacteria.
Doktycz and his team do not know the mechanism of toxicity but believe particle surface coatings play a key role. Analysis of the nanoparticles produced by S. oneidensis show that they are coated with an as-yet-unknown material, although the researchers say it is characteristic of a protein. 'These coatings, it turns out, can have quite an effect on their apparent toxicity,' says Doktycz.
Images showing the interaction of E. coli with silver nanoparticles. Top:
© Environ. Sci. Technol.
But there are many questions still unanswered. The team wants to identify the peptide coating as well as the mechanisms of synthesis and toxicity. 'Because bacteria are so complex, it's hard to infer exactly what's going on,' says team member says Anil Suresh. However ,they hope by choosing well-characterised organisms, they have given themselves a head start.
Kelvin Gregory, an environmental microbiologist at Carnegie Mellon University, Pennsylvania, US, thinks the team's findings will have great impact on future research. 'It's somewhat controversial in that prior work has shown that both natural and engineered polymer coatings on a range of nanoparticles greatly reduce growth inhibition and toxicity of nanoparticles in microorganisms,' he says. 'Taken with previous work, this research suggests there are means by which particles can be designed for reduced toxicity in the environment.'
'One of the take home messages is that generic statements about toxicity and nanomaterials just cannot be made,' claims Doktycz. 'If we can start gaining a molecular level understanding we might have a more informed approach for effective uses of nanomaterials.'
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A K Suresh et al, Environ. Sci. Technol., 2010, DOI: 10.1021/es903684r
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