Issue 4, 2002

Bacterial chromosome extraction and isolation

Abstract

We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.

Article information

Article type
Paper
Submitted
15 Aug 2002
Accepted
24 Oct 2002
First published
07 Nov 2002

Lab Chip, 2002,2, 207-212

Bacterial chromosome extraction and isolation

C. Prinz, J. O. Tegenfeldt, R. H. Austin, E. C. Cox and J. C. Sturm, Lab Chip, 2002, 2, 207 DOI: 10.1039/B208010A

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