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Paper
Analyst, 2005, 130, 1404 - 1409, DOI: 10.1039/b509618a
Flow injection fluorimetric determination of
-estradiol using a molecularly imprinted polymerJuan C. Bravo, Pilar Fernández and Jesús S. Durand
The steroidal compound 17
-estradiol (E2) is a major estrogen now widely studied because of its potential endocrine disruption effects. The present paper describes a simple, rapid, flow injection (FI) method for the fluorimetric determination of -estradiol involving a molecularly imprinted polymer (MIP) packed into a microcolumn. Microwave-assisted extraction, a relatively new technique, was used to help remove the template from the MIP; this improved extraction efficiency. The optimization of the FI parameters and the intrinsic fluorescence of -estradiol allowed its direct determination without a derivatization step. When the possible interference of other estrogens was examined, the system displayed excellent specificity for -estradiol; no response to the natural estrogens estrone and estriol and the xenoestrogen bisphenol A was recorded. Under optimum experimental conditions, linear calibration graphs were obtained from 4 to 80 µg L–1 with a detection limit of 1.12 µg L–1.
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