Issue 10, 2009
From the journal:

Photochemical & Photobiological Sciences

Photophysical characterization of oligopyrene modules for DNA-based nanosystems†

Victor A. Galievsky,a   Vladimir L. Malinovskii,b   Alexander S. Stasheuski,a   Florent Samain,b   Klaas A. Zachariasse,c   Robert Häner*b  and  Vladimir S. Chirvony*ad  

* Corresponding authors

a B.I. Stepanov's Institute of Physics, National Academy of Sciences of Belarus, Nezavisimosti ave. 68, Minsk, Belarus

b Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Bern, Switzerland
E-mail: robert.haener@ioc.unibe.ch

c Max-Planck-Institut für biophysikalische Chemie, Spektroskopie und Photochemische Kinetik, Göttingen, Germany

d Instituto de Ciencia de los Materiales, Universidad de Valencia, P.O. Box 22085, Valencia, Spain
E-mail: vladimir.chyrvony@uv.es

Abstract

The photophysics of free pyrenedicarboxamide (Py-DCA) in solution as well as of single-stranded and double-stranded oligonucleotides (ss and ds ONs) containing 1–7 pyrene building blocks per strand were studied by steady-state and time-resolved fluorescence spectroscopy. It was found that the fluorescence quantum yield ΦF of free Py-DCA chromophore in solution is rather high (ΦF = 0.44). However, after incorporation of the chromophore into a ss ON the monomeric chromophore fluorescence is quenched more than 40-fold due to electron-transfer reactions with ON bases. An increase of the number n of neighboring pyrenes in an ON results in ΦF growth up to 0.25 at n = 6. Starting from n = 2, all fluorescence belongs mainly to excimer formed by pyrene chromophores. Sections composed of multiple pyrenes may be considered as robust functional entities that may serve as independent modules in DNA-based, functional nano-architectures.

Graphical abstract: Photophysical characterization of oligopyrene modules for DNA-based nanosystems

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B9PP00016J
Article type
Paper
Submitted
08 Jun 2009
Accepted
13 Jul 2009
First published
07 Aug 2009

Photochem. Photobiol. Sci., 2009,8, 1448-1454

Permissions

Request permissions

Photophysical characterization of oligopyrene modules for DNA-based nanosystems

V. A. Galievsky, V. L. Malinovskii, A. S. Stasheuski, F. Samain, K. A. Zachariasse, R. Häner and V. S. Chirvony, Photochem. Photobiol. Sci., 2009, 8, 1448 DOI: 10.1039/B9PP00016J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements