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The interactive lab primer - column chromatography

Description

The separation of mixtures produced in chemical reactions is often carried out by passing the mixture through a stationary phase of silica held in a column.  Solvents move compounds at different rates through the silica allowing them to be separated into fractions by collecting the emerging solvent. This can be carried out by allowing the solvent to flow under gravity or under a moderate pressure to increase the solvent flow rate ('flash chromatography'). The fractions are usually analysed by TLC in order to identify which components of the mixture they contain.

Type of Activity

:
demonstration

Audience

:
TeacherStudent

Age Group

:
16 to Undergraduate
column-chromatography

Video instructions on how to separate a mixture of compounds using column chromatography

ADDITIONAL INFORMATION

This resource is part of the interactive lab primer - lab techniques collection.

See more lab techniques or visit the interactive lab primer home.