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Substance:Rhodamine
From Learn Chemistry Wiki
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- Main ChemSpider page
- Molecular formula: C28H31N2O3
- Molar mass: 443.557
- CAS Registry Number: Not available
- Appearance: Not available
- Melting point: Not available
- Boiling point: Not available
- Solubility: Not available
- Safety sheet: Not available
- Spectra: NMRShiftDB 13C NMR, also check on SDBS. Add Spectra (Help).
From Wikipedia
Rhodamine () is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine dyes are generally toxic, and are soluble in water, methanol and ethanol.
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Rhodamine B () is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium.
Rhodamine B is tunable around 610 nm when used as a laser dye. Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol, 1.0, and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent.
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References
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