Issue 8, 2003
From the journal:

Analyst

Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

Qing Deng,a   Lisa M. Kauri,a   Wei-Jun Qian,a   Gabriella M. Dahlgrena  and  Robert T. Kennedy*a  

* Corresponding authors

a Department of Chemistry and Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109-1055, USA

Abstract

A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 µm inner diameter by 25 cm long) packed with 7 µm reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content.

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Article information

DOI
https://doi.org/10.1039/B303949H
Article type
Paper
Submitted
08 Apr 2003
Accepted
07 Jun 2003
First published
14 Jul 2003

Analyst, 2003,128, 1013-1018

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Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

Q. Deng, L. M. Kauri, W. Qian, G. M. Dahlgren and R. T. Kennedy, Analyst, 2003, 128, 1013 DOI: 10.1039/B303949H

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