Issue 3, 2001
From the journal:

Analyst

Electroanalytical exploitation of quinone–thiol interactions: application to the selective determination of cysteine

Geraldine Hignett,a   Sarah Threlfell,a   Andrew J. Wain,a   Nathan S. Lawrence,a   Shelley J. Wilkins,a   James Davis,a   Richard G. Compton*a  and  Marco F. Cardosib  

* Corresponding authors

a Physical and Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK
E-mail: richard.compton@chemistry.ox.ac.uk

b Department of Biological Sciences, University of Paisley, Paisley, UK

Abstract

Square wave voltammetry was applied to the detection of cysteine through the use of an indirect assay that exploits the reaction of the thiol with a quinone indicator. Voltammetric discrimination between unreacted quinone and the corresponding quinone–cysteine adduct is possible with clear resolution of the latter peak providing a linear response from 5 to 47 μM. The selectivity of the approach was assessed with no interference from cystine, lysine, paracetamol or 4-aminophenol. The response recorded in the presence of a massive excess of ascorbic acid was also investigated and the integrity of the approach confirmed. The effects of other sulfhydryl thiols, homocysteine and glutathione, were also assessed and found to present no appreciable change in the voltammetric profile. The practical utility of the approach was investigated through examining the response to cysteine in urine.

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Article information

DOI
https://doi.org/10.1039/B008616I
Article type
Paper
Submitted
25 Oct 2000
Accepted
18 Jan 2001
First published
19 Feb 2001

Analyst, 2001,126, 353-357

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Electroanalytical exploitation of quinone–thiol interactions: application to the selective determination of cysteine

G. Hignett, S. Threlfell, A. J. Wain, N. S. Lawrence, S. J. Wilkins, J. Davis, R. G. Compton and M. F. Cardosi, Analyst, 2001, 126, 353 DOI: 10.1039/B008616I

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