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Communication
Chem. Commun., 2004, 2412 - 2413, DOI: 10.1039/b407924h
Intein-mediated purification of a recombinantly expressed peptide
John A. Pezza, Karen N. Allen and Dean R. Tolan
A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.
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