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Chem. Commun., 2010, 46, 469 - 471, DOI: 10.1039/b915843j

FLAG tagging by CuAAC and nanogram-scale purification of the target protein for a bioactive metabolite involved in circadian rhythmic leaf movement in Leguminosae

Yoshiyuki Manabe, Makoto Mukai, Satoko Ito, Nobuki Kato and Minoru Ueda

We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 × 105 differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.

Graphical abstract image for this article (ID: b915843j)