Issue 8, 2004
From the journal:

Journal of Materials Chemistry

Synthesis and characterization of multi-functional nanoparticles possessing magnetic, up-conversion fluorescence and bio-affinity properties

Huachang Lu,ab   Guangshun Yi,ac   Shuying Zhao,b   Depu Chen,*ab   Liang-Hong Guo*b  and  Jing Chengbd  

* Corresponding authors

a Department of Chemistry, Tsinghua University, Beijing, P. R. China
E-mail: chendp@chem.tsinghua.edu.cn
Fax: (86) 10-62782485
Tel: (86) 10-62781691

b National Research Engineering Center for Beijing Biochip Technology, 18 Life Science Parkway, Changping District, Beijing, P. R. China
E-mail: LHGuo@capitalbiochip.com
Fax: (86) 10-80726898
Tel: (86) 10-80726775

c School of Materials Science & Engineering, Shandong University of Technology, Zibo, Shandong, P. R. China

d Department of Biological Sciences and Biotechnology, and State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, P. R. China

Abstract

Multi-functional nanoparticles possessing magnetic, up-conversion fluorescence and bio-affinity properties were synthesized and characterized. The particles have a core/shell structure. Iron oxide nanoparticles of 5–15 nm diameter were synthesized as the magnetic core. The core was covered with ytterbium and erbium co-doped sodium yttrium fluoride (NaYF4:Yb,Er), an efficient infrared-to-visible up-conversion phosphor. The phosphor shell was prepared by co-precipitation of the rare-earth metal salts with fluoride in the presence of EDTA and the magnetic nanoparticle. After the magnetic/fluorescent hybrid particle was coated with SiO2 and activated with glutaraldehyde, streptavidin was immobilized on the particle. The magnetic/fluorescent nanoparticles were found by transmission electron microscopy to be well-dispersed spherical particles with an average diameter of 68 nm. Both energy dispersive X-ray microanalysis and X-ray fluorescence spectra revealed the existence of iron in the particle. Measurements performed on a vibrating sample magnetometer obtained a strong magnetic response for the particle and fluorescence measurements demonstrated its up-conversion property. X-Ray diffraction analysis suggests the phosphor shell has the same structure as the pure NaYF4:Yb,Er nanoparticles we prepared in a previous study (G. S. Yi, H. C. Lu, S. Y. Zhao, Y. Ge, W. J. Yang, L. H. Guo, D. P. Chen and J. Cheng, submitted). Streptavidin-coated magnetic/fluorescent particles were found to bind specifically to a glass slide spotted with biotinylated IgG and emit up-conversion fluorescence, confirming the successful coating of the protein and retention of its optical activity and bio-affinity.

Graphical abstract: Synthesis and characterization of multi-functional nanoparticles possessing magnetic, up-conversion fluorescence and bio-affinity properties

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Article information

DOI
https://doi.org/10.1039/B315103D
Article type
Paper
Submitted
21 Nov 2003
Accepted
13 Feb 2004
First published
04 Mar 2004

J. Mater. Chem., 2004,14, 1336-1341

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Synthesis and characterization of multi-functional nanoparticles possessing magnetic, up-conversion fluorescence and bio-affinity properties

H. Lu, G. Yi, S. Zhao, D. Chen, L. Guo and J. Cheng, J. Mater. Chem., 2004, 14, 1336 DOI: 10.1039/B315103D

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