Issue 6, 2006
From the journal:

Lab on a Chip

Timing controllable electrofusion device for aqueous droplet-based microreactors†

Wei-Heong Tana  and  Shoji Takeuchi*a  

* Corresponding authors

a CIRMM/IIS, The University of Tokyo, Institute of Industrial Science, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
E-mail: takeuchi@iis.u-tokyo.ac.jp
Fax: 81-3-5452-6649
Tel: 81-3-5452-6650

Abstract

This paper describes an electrofusion device for controlling the precise moment of fusion between droplets by applying an electric field. This device allows (i) accurate determination of the start of chemical/biological reactions, (ii) minimum contact of reactants with channel walls—eliminating surface absorption problems, (iii) easy fabrication and (iv) continuous observation of initiated reaction. We demonstrated the fusion of β-galactosidase and fluorescein di-β-D-galactopyranoside (FDG) droplets, and observed the enzymatic reaction using fluorescence microscopy. In addition, sequential fusion of pico-litre droplets was also accomplished.

Graphical abstract: Timing controllable electrofusion device for aqueous droplet-based microreactors

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Article information

DOI
https://doi.org/10.1039/B517178D
Article type
Paper
Submitted
05 Dec 2005
Accepted
20 Mar 2006
First published
31 Mar 2006

Lab Chip, 2006,6, 757-763

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Timing controllable electrofusion device for aqueous droplet-based microreactors

W. Tan and S. Takeuchi, Lab Chip, 2006, 6, 757 DOI: 10.1039/B517178D

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