Issue 3, 2007
From the journal:

Lab on a Chip

Integrated microelectronic device for label-free nucleic acid amplification and detection†

Chih-Sheng Johnson Hou,a   Michel Godin,b   Kristofor Payer,c   Raj Chakrabartidf  and  Scott R. Manalis*be  

* Corresponding authors

a Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

b Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
E-mail: scottm@media.mit.edu

c Microsystems Technology Laboratories, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

d Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

e Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

f Department of Chemistry, Columbia University, New York, NY 10025, USA

Abstract

We present an integrated microelectronic device for amplification and label-free detection of nucleic acids. Amplification by polymerase chain reaction (PCR) is achieved with on-chip metal resistive heaters, temperature sensors, and microfluidic valves. We demonstrate a rapid thermocycling with rates of up to 50 °C s−1 and a PCR product yield equivalent to that of a bench-top system. Amplicons within the PCR product are detected by their intrinsic charge with a silicon field-effect sensor. Similar to existing optical approaches with intercalators such as SYBR Green, our sensing approach can directly detect standard double-stranded PCR product, while in contrast, our sensor does not require labeling reagents. By combining amplification and detection on the same device, we show that the presence or absence of a particular DNA sequence can be determined by converting the analog surface potential output of the field-effect sensor to a simple digital true/false readout.

Graphical abstract: Integrated microelectronic device for label-free nucleic acid amplification and detection

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Article information

DOI
https://doi.org/10.1039/B617082J
Article type
Paper
Submitted
22 Nov 2006
Accepted
18 Jan 2007
First published
08 Feb 2007

Lab Chip, 2007,7, 347-354

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Integrated microelectronic device for label-free nucleic acid amplification and detection

C. Johnson Hou, M. Godin, K. Payer, R. Chakrabarti and S. R. Manalis, Lab Chip, 2007, 7, 347 DOI: 10.1039/B617082J

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