Issue 3, 2010
From the journal:

Lab on a Chip

Multilayers of hydrogels loaded with microparticles: a fast and simple approach for microarray manufacturing†

Marta Bally,a   Janos Vörös*a  and  Shoji Takeuchib  

* Corresponding authors

a Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH and University Zurich, 8092 Zurich, Switzerland
E-mail: janos.voros@biomed.ee.ethz.ch
Fax: +4144 632 11 93
Tel: +4144 632 59 03

b Center for International Research on MicroMechanotronics (CIRMM), Institute of Industrial Science (IIS), The University of Tokyo, Tokyo, Japan

Abstract

We present a novel and simple approach towards the creation of arrays of biomolecules for the multiplexed detection of biological interactions. Microarrays were obtained by cutting stacked layers of biofunctionalized polystyrene particle layers embedded in a permeable agarose matrix. Microparticles were therefore the vehicles for biorecognition. The three-dimensional constructs were obtained by consecutive dipping steps in a pre-gel solution. Our strategy enables the rapid manufacturing of a large number of array copies in a flexible manner and without any specialized instrumentation. Model binding assays for the detection of rabbit and mouse IgG were performed as a proof of concept using a fluorescence microscope for read-out. The limits of detection were in the low picomolar range for the sandwich assay while 1 IgG out of 50 000 background proteins could be detected in a reverse phase assay. Thus, without any assay optimization, sensitivities comparable to the ones usually observed for standard fluorescence-based assays were achieved with the particle/hydrogel array.

Graphical abstract: Multilayers of hydrogels loaded with microparticles: a fast and simple approach for microarray manufacturing

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Article information

DOI
https://doi.org/10.1039/B916071J
Article type
Paper
Submitted
05 Aug 2009
Accepted
14 Oct 2009
First published
23 Nov 2009

Lab Chip, 2010,10, 372-378

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Multilayers of hydrogels loaded with microparticles: a fast and simple approach for microarray manufacturing

M. Bally, J. Vörös and S. Takeuchi, Lab Chip, 2010, 10, 372 DOI: 10.1039/B916071J

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