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Mol. BioSyst., 2007, 3, 381 - 391, DOI: 10.1039/b617204k
Imaging proteins in vivo using fluorescence lifetime microscopy
Frederic Festy, Simon M. Ameer-Beg, Tony Ng and Klaus Suhling
Fluorescence lifetime imaging (FLIM) represents a key optical technique for imaging proteins and protein interaction in vivo. We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development.

