_tcm18-15276.gif )
Research at the interface between chemistry and the -omic sciences and systems biology.
Paper
Mol. BioSyst., 2006, 2, 305 - 311, DOI: 10.1039/b604362n
An altered oxidant defense system in red blood cells affects their ability to release nitric oxide-stimulating ATP
Jamie Carroll, Madushi Raththagala, Wasanthi Subasinghe, Stacy Baguzis, Teresa D'amico Oblak, Paul Root and Dana Spence
A novel microflow technique is used to demonstrate that a weakened oxidant defense system found in diabetic erythrocytes leads to decreased levels of deformation-induced release of adenosine triphosphate (ATP) from erythrocytes. Addition of an oxidant to rabbit erythrocytes resulted in a 63% decrease in deformation-induced ATP release before eventually recovering to a value that was statistically equivalent to the initial value. Inhibition of glucose-6-phosphate dehydrogenase prevents recovery from the oxidant attack. Finally, results indicated that the ATP release from the erythrocytes of type II diabetics (91 nM ± 10 nM) was less than half of that measured from the erythrocytes of healthy controls (190 ± 10 nM). These data suggest that the antioxidant status of erythrocytes is a critical determinant in the ability of these cells to release ATP, a known nitric oxide stimulus.
RSS feed