Issue 6, 2008
From the journal:

Molecular BioSystems

A simple strategy for the creation of a recombinant lectin microarray†‡

Ku-Lung Hsu,a   Jeffrey C. Gildersleevec  and  Lara K. Mahal*b  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station, A5300, Austin, USA

b Department of Chemistry and Biochemistry, Center for Systems and Synthetic Biology and Institute for Cellular and Molecular Biology, University of Texas at Austin, 1 University Station, A5300, Austin, USA
E-mail: lmahal@cm.utexas.edu
Fax: +1 (512) 471-8696
Tel: +1 (512) 471-2318

c Laboratory of Medicinal Chemistry, Center for Cancer Research, NCI-Frederick, 376 Boyles Street, Building 376, Room 109, Frederick, USA

Abstract

Glycomics, i.e. the high-throughput analysis of carbohydrates, has yet to reach the level of ease and import of its counterparts, genomics and proteomics, due to the difficulties inherent in carbohydrate analysis. The advent of lectin microarray technology addresses many of these problems, providing a straightforward approach for glycomic analysis. However, current microarrays are limited to the available lectin set, which consists mainly of plant lectins isolated from natural sources. These lectins have inherent problems including inconsistent activity and availability. Also, many plant lectins are glycosylated, complicating glycomic evaluation of complex samples, which may contain carbohydrate-binding proteins. The creation of a recombinant, well-defined lectin set would resolve many of these issues. Herein, we describe an efficient strategy for the systematic creation of recombinant lectins for use in microarray technology. We present a small panel of simple-to-purify bacterially-derived lectins that show reliable activity and define their binding specificities by both carbohydrate microarray and ELISA. We utilize this panel to create a recombinant lectin microarray that is able to distinguish glycopatterns for both proteins and cell samples. This work opens the door to the establishment of a vast set of defined lectinsvia high-throughout approaches, advancing lectin microarray technology for glycomic analysis.

Graphical abstract: A simple strategy for the creation of a recombinant lectin microarray

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B800725J
Article type
Paper
Submitted
16 Jan 2008
Accepted
27 Mar 2008
First published
14 Apr 2008

Mol. BioSyst., 2008,4, 654-662

Permissions

Request permissions

A simple strategy for the creation of a recombinant lectin microarray

K. Hsu, J. C. Gildersleeve and L. K. Mahal, Mol. BioSyst., 2008, 4, 654 DOI: 10.1039/B800725J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements