Issue 7, 2010
From the journal:

Molecular BioSystems

Ultrasensitive detection of antibodies using a new Tus–Ter-lock immunoPCR system†

Isabelle Morin,a   Nicholas E. Dixonb  and  Patrick M. Schaeffer*a  

* Corresponding authors

a Comparative Genomics Centre, School of Pharmacy & Molecular Sciences, DB 21, James Cook Drive, James Cook University, Townsville, Australia
E-mail: Patrick.Schaeffer@jcu.edu.au
Fax: +61 (0)7 47816078
Tel: +61 (0)7 47814448

b School of Chemistry, University of Wollongong, Wollongong, Australia

Abstract

A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus–Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.

Graphical abstract: Ultrasensitive detection of antibodies using a new Tus–Ter-lock immunoPCR system

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Article information

DOI
https://doi.org/10.1039/C002163F
Article type
Communication
Submitted
01 Feb 2010
Accepted
26 Feb 2010
First published
08 Mar 2010

Mol. BioSyst., 2010,6, 1173-1175

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Ultrasensitive detection of antibodies using a new Tus–Ter-lock immunoPCR system

I. Morin, N. E. Dixon and P. M. Schaeffer, Mol. BioSyst., 2010, 6, 1173 DOI: 10.1039/C002163F

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