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Paper

Org. Biomol. Chem., 2008, 6, 3837 - 3842, DOI: 10.1039/b810106j

PNA based artificial nucleases displaying catalysis with turnover in the cleavage of a leukemia related RNA model

Merita Murtola and Roger Strömberg

Several peptide nucleic acid based artificial nucleases (PNAzymes) are designed to create a bulge in the target RNA, which is a short model of the leukemia related bcr/abl mRNA. The target RNA is cleaved by the PNAzymes with a half-life of down to 11 h (using a 1 : 1 ratio of PNA-conjugate to target) and only upon base-pairing with the substrate. The PNA based systems are also shown to act in a catalytic fashion with turnover of substrate and are thus the first reported peptide nucleic acid based artificial RNA-cleaving enzymes.

Graphical abstract image for this article (ID: b810106j)