Issue 13, 2007
From the journal:

Organic & Biomolecular Chemistry

Identification of emissive lanthanide complexes suitable for cellular imaging that resist quenching by endogenous anti-oxidants

Robert A. Poole,a   Craig P. Montgomery,a   Elizabeth J. New,a   Aileen Congreve,a   David Parker*a  and  Mauro Bottab  

* Corresponding authors

a Department of Chemistry, Durham University, South Road, Durham, UK
E-mail: david.parker@dur.ac.uk

b Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale “Amadeo Avogadro”, Via Bellini 25/G, Alessandria, Italy

Abstract

Excited state quenching by urate and ascorbate of selected europium and terbium(III) macrocyclic complexes has been assessed and related to the ease of complex visualisation by optical microscopy inside various living cells, e.g. CHO, COS and NIH 3T3. It is the relative insensitivity of certain sterically encumbered complexes to dynamic quenching by urate that favours their usage for in cellulo applications. Non-covalent binding of the complex by protein also shields the excited lanthanide(III) ion from collisional quenching; this effect is most marked for a cationic triamide complex, [Ln.1]3+, consistent with its ease of visualisation by luminescence microscopy.

Graphical abstract: Identification of emissive lanthanide complexes suitable for cellular imaging that resist quenching by endogenous anti-oxidants

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Article information

DOI
https://doi.org/10.1039/B705943D
Article type
Paper
Submitted
19 Apr 2007
Accepted
14 May 2007
First published
31 May 2007

Org. Biomol. Chem., 2007,5, 2055-2062

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Identification of emissive lanthanide complexes suitable for cellular imaging that resist quenching by endogenous anti-oxidants

R. A. Poole, C. P. Montgomery, E. J. New, A. Congreve, D. Parker and M. Botta, Org. Biomol. Chem., 2007, 5, 2055 DOI: 10.1039/B705943D

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