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Org. Biomol. Chem., 2007, 5, 2458 - 2463, DOI: 10.1039/b706507h
Efficient chemoenzymatic synthesis of biotinylated human serum albumin–sialoglycoside conjugates containing O-acetylated sialic acids
Hai Yu, Harshal A. Chokhawala, Ajit Varki and Xi Chen
Sialyl Tn (STn) and sialyl lactoside derivatives containing O-acetylated sialic acid residues have been chemoenzymatically synthesized using a one-pot three-enzyme system and conjugated to biotinylated human serum albumin (HSA) using an adipic acid para-nitrophenyl ester coupling reagent. This approach provides an efficient and general protocol for preparing carbohydrate–protein conjugates containing base-sensitive groups.
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